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A miniaturised integrated biophotonic point-of-care genotyping system
journal contribution
posted on 2020-07-09, 15:48 authored by John M Girkin, Mazher MohammedMazher Mohammed, Elizabeth M EllisThis paper reports the development of a novel genotyping device specifically designed for point-of-care applications. As the results of the human genome project are applied to clinical practice there is an increasing requirement for simple to operate high-speed, potentially low-cost genotyping devices for use in the clinic. The aim of such devices is not to specifically detect a full gene sequence but to monitor the presence of specific Single Nucleotide Polymorphisms (SNPs). The instrument is designed to fulfil this specific clinical requirement. Using a FRET-based assay the instrument completes a full PCR process and then performs a melting point test to determine the exact SNPs present in the sample. Results are presented in which the instrument produces results within 18 min based upon saliva samples provided by the patient. The paper also reports successful results both with purified DNA samples and saliva-based samples which were taken from subjects after experiments deliberately aimed at confusing the instrument.
Funding
Engineering and Physical Sciences Research Council (EPSRC)
History
School
- Design
Published in
Faraday DiscussionsVolume
149Pages
115 - 123Publisher
Royal Society of Chemistry (RSC)Version
- VoR (Version of Record)
Rights holder
© The Royal Society of ChemistryPublisher statement
This paper was accepted for publication in the journal Faraday Discussions and the definitive published version is available at https://doi.org/10.1039/c005271j.Acceptance date
2010-07-19Publication date
2010-09-24Copyright date
2011ISSN
1359-6640eISSN
1364-5498Publisher version
Language
- en