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A new freeware tool for image processing and its application to high speed LA-ICP-MS imaging

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journal contribution
posted on 24.04.2019, 10:45 by Amy ManaghAmy Managh, Peter Reid
A new standalone, non-commercial software application has been developed to enable rapid generation of images from laser ablation – inductively coupled plasma – mass spectrometry data. The software locates peaks within raw data files and uses the peak locations to segment the data at appropriate intervals, enabling conversion of the data into a matrix of colour coded pixels. The app was developed in order to process baseline separated peaks produced by the latest generation of low-dispersion laser ablation systems, but it is equally applicable to rastered imaging using alternative laser ablation platforms. The peak recognition feature also has applicability to single cell and single particle ICP-MS applications. The app was demonstrated for the imaging of zinc in a stained section of rat retina tissue at lateral resolutions down to 1 µm. Less than two minutes was required for conversion of the raw data into a 60,000 pixel image.

History

School

  • Science

Department

  • Chemistry

Published in

Journal of Analytical Atomic Spectrometry

Volume

34

Issue

7

Pages

1369-1373

Citation

MANAGH, A.J. and REID, P., 2019. A new freeware tool for image processing and its application to high speed LA-ICP-MS imaging. Journal of Analytical Atomic Spectrometry, 34 (7), pp.1369-1373.

Publisher

© Royal Society of Chemistry

Version

AM (Accepted Manuscript)

Publisher statement

This paper was accepted for publication in the journal Journal of Analytical Atomic Spectrometry and the definitive published version is available at https://doi.org/10.1039/c9ja00082h.

Acceptance date

18/04/2019

Publication date

2019-04-18

ISSN

0267-9477

eISSN

1364-5544

Language

en

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