A new long-wavelength fluorigenic substrate for alkaline.pdf (53.4 kB)
Download fileA new long-wavelength fluorigenic substrate for alkaline phosphatase: synthesis and characterisation
journal contribution
posted on 2016-08-03, 08:42 authored by G. Hussain Sarpara, Sijung HuSijung Hu, Derek A. Palmer, Martin T. French, Mark Evans, James N. MillerNaphthofluorescein diphosphate has been synthesised from
the parent dye, and shown to be an attractive longwavelength
alternative to other fluorigenic substrates for the
determination of alkaline phosphatase. Its application to the
determination of theophylline, an inhibitor of this enzyme,
has been demonstrated. The optimum excitation wavelength
of the hydrolysis product naphthofluorescein has been found
to depend on the presence of additives such as cyclodextrins
and (3-[3-cholamidopropyl]-dimethylamino)-1-propane sulfonate
(CHAPS): such effects can be used to raise the
excitation wavelength to match the output of a 635 nm diode
laser in a simple and sensitive fluorescence detector.
Funding
G.H.S. thanks the Government of Pakistan for financial support.
History
School
- Mechanical, Electrical and Manufacturing Engineering
Published in
Analytical CommunicationsPages
. 19 - 20Citation
SARPARA, G. ... et al., 1999. A new long-wavelength fluorigenic substrate for alkaline phosphatase: synthesis and characterisation. Analytical Communications, 36, pp.19-20.Publisher
© Royal Society of ChemistryVersion
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
1999ISSN
1359-7337eISSN
1364-5536Publisher version
Language
- en