2015_JPD_protocol_v2.pdf (1.52 MB)
A reference protocol for comparing the biocidal properties of gas plasma generating devices
journal contributionposted on 2015-12-01, 12:18 authored by Alexander H. Shaw, P. Seri, Carlo A. Borghi, Gilbert Shama, Felipe IzaFelipe Iza
The growing interest in the use of non-thermal, atmospheric pressure gas plasmas for decontamination purposes has resulted in a multiplicity of plasma- generating devices. There is currently no uni versally approved method of comparing the biocidal performance of such devices and in the work described here spores of the Gram positive bacterium Bacillus subtilis (ATCC 6633) are proposed as a suitable reference biological agent. In order to achieve consistency in the form in which the biological agent in question is presented to the plasma, a polycarbonate membrane loaded with a monolayer of spores is proposed. The advantages of the proposed protocol are evaluated by comparing inactivation tests in which an alternative microorganism (methicillin resistant Staphylococcus aureus - MRSA) and the widely-used sample preparation technique of directly pipetting cell suspensions onto membranes are employed. In all cases, inactivation tests with either UV irradiation or plasma exposure were more reproducible when the proposed protocol was followed.
This work was supported by the UK Engineering and Physical Sciences Research Council (EPSRC), Loughborough University and the University of Bologna.
- Mechanical, Electrical and Manufacturing Engineering
Published inJournal of Physics D: Applied Physics
Pages1 - 1 (9)
CitationSHAW, A. ...et al., 2015. A reference protocol for comparing the biocidal properties of gas plasma generating devices. Journal of Physics D: Applied Physics, 48(48).
Publisher© IOP Publishing
- AM (Accepted Manuscript)
Publisher statementThis work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/
NotesThis is an author-created, un-copyedited version of an article accepted for publication in Journal of Physics D: Applied Physics. The publisher is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at http://dx.doi.org/10.1088/0022-3727/48/48/484001.