A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film
journal contributionposted on 14.07.2014, 12:44 authored by Alexander D. Edwards, Nuno Reis, Nigel K.H. Slater, Malcolm R. Mackley
We present a simple device for multiplex quantitative enzyme-linked immunosorbant assays (ELISA) made from a novel melt-extruded microcapillary film (MCF) containing a parallel array of 200μm capillaries along its length. To make ELISA devices different protein antigens or antibodies were immobilised inside individual microcapillaries within long reels of MCF extruded from fluorinated ethylene propylene (FEP). Short pieces of coated film were cut and interfaced with a pipette, allowing sequential uptake of samples and detection solutions into all capillaries from a reagent well. As well as being simple to produce, these FEP MCF devices have excellent light transmittance allowing direct optical interrogation of the capillaries for simple signal quantification. Proof of concept experiments demonstrate both quantitative and multiplex assays in FEP MCF devices using a standard direct ELISA procedure and read using a flatbed scanner. This new multiplex immunoassay platform should find applications ranging from lab detection to point-of-care and field diagnostics. This journal is © The Royal Society of Chemistry 2011.
ADE and NMR are grateful to the Technology Strategy Board, the EPSRC and the European Commission (Marie Curie programme) for financial support.
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