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A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film

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journal contribution
posted on 14.07.2014, 12:44 by Alexander D. Edwards, Nuno Reis, Nigel K.H. Slater, Malcolm R. Mackley
We present a simple device for multiplex quantitative enzyme-linked immunosorbant assays (ELISA) made from a novel melt-extruded microcapillary film (MCF) containing a parallel array of 200μm capillaries along its length. To make ELISA devices different protein antigens or antibodies were immobilised inside individual microcapillaries within long reels of MCF extruded from fluorinated ethylene propylene (FEP). Short pieces of coated film were cut and interfaced with a pipette, allowing sequential uptake of samples and detection solutions into all capillaries from a reagent well. As well as being simple to produce, these FEP MCF devices have excellent light transmittance allowing direct optical interrogation of the capillaries for simple signal quantification. Proof of concept experiments demonstrate both quantitative and multiplex assays in FEP MCF devices using a standard direct ELISA procedure and read using a flatbed scanner. This new multiplex immunoassay platform should find applications ranging from lab detection to point-of-care and field diagnostics. This journal is © The Royal Society of Chemistry 2011.

Funding

ADE and NMR are grateful to the Technology Strategy Board, the EPSRC and the European Commission (Marie Curie programme) for financial support.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Lab on a Chip - Miniaturisation for Chemistry and Biology

Volume

11

Issue

24

Pages

4267 - 4273

Citation

EDWARDS, A.D. ... et al, 2011. A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film. Lab on a Chip, 11 (24), pp.4267-4273.

Publisher

© The Royal Society of Chemistry

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2011

Notes

This article was published in the journal Lab on a Chip [© Royal Society of Chemistry] and the definitive version is available at: http://dx.doi.org/10.1039/c0lc00357c

ISSN

1473-0197

eISSN

1473-0189

Language

en

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