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A survey to evaluate parameters governing the selection and application of extracellular vesicle isolation methods

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posted on 2023-05-05, 14:26 authored by Soraya WilliamsSoraya Williams, Aveen Jalal Jalal, Mark LewisMark Lewis, Owen DaviesOwen Davies
Extracellular vesicles (EVs) continue to gain interest across the scientific community for diagnostic and therapeutic applications. As EV applications diversify, it is essential that researchers are aware of challenges, in particular the compatibility of EV isolation methods with downstream applications and their clinical translation. We report outcomes of the first cross-comparison study looking to determine parameters (EV source, starting volume, operator experience, application and implementation parameters such as cost and scalability) governing the selection of popular EV isolation methods across disciplines. Our findings highlighted an increased clinical focus, with 36% of respondents applying EVs in therapeutics and diagnostics. Data indicated preferential selection of ultracentrifugation for therapeutic applications, precipitation reagents in clinical settings and size exclusion chromatography for diagnostic applications utilising biofluids. Method selection was influenced by operator experience, with increased method diversity when EV research was not the respondents primary focus. Application and implementation criteria were indicated to be major influencers in method selection, with UC and SEC chosen for their abilities to process large and small volumes, respectively. Overall, we identified parameters influencing method selection across the breadth of EV science, providing a valuable overview of practical considerations for the effective translation of research outcomes.

Funding

Engineering and Physical Sciences Research Council (EPSRC)

Medical Research Council (MRC) Centre of Doctoral Training

History

School

  • Sport, Exercise and Health Sciences

Published in

Journal of Tissue Engineering

Volume

14

Publisher

SAGE Publications

Version

  • VoR (Version of Record)

Rights holder

© The Author(s)

Publisher statement

This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage)

Acceptance date

2023-01-19

Publication date

2023-03-08

Copyright date

2023

ISSN

2041-7314

eISSN

2041-7314

Language

  • en

Depositor

Deposit date: 5 May 2023

Article number

ARTN 20417314231155114

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