Arthur et al_3-MX_FAIMS_Manuscript_accepted version 2016.pdf (833.93 kB)

Analysis of supramolecular complexes of 3-methylxanthine with field asymmetric waveform ion mobility spectrometry combined with mass spectrometry

Download (833.93 kB)
journal contribution
posted on 15.03.2016, 11:29 by Kayleigh Arthur, Gary Eiceman, Jim Reynolds, Colin Creaser
Miniaturised field asymmetric waveform ion mobility spectrometry (FAIMS), combined with mass spectrometry (MS), has been applied to the study of self-assembling, non-covalent supramolecular complexes of 3-methylxanthine (3-MX) in the gas phase. 3-MX forms stable tetrameric complexes around an alkali metal (Na+, K+) or ammonium cation, to generate a diverse array of complexes with single and multiple charge states. Complexes of (3-MX)n observed include: singly charged complexes where n = 1-8 and 12 and doubly charged complexes where n = 12-24. The most intense ions are those associated with multiples of tetrameric units, where n = 4, 8, 12, 16, 20, 24. The effect of dispersion field on the ion intensities of the self-assembled complexes indicates some fragmentation of higher order complexes within the FAIMS electrodes (in-FAIMS dissociation), as well as in-source collision induced dissociation within the mass spectrometer. FAIMS-MS enables charge state separation of supramolecular complexes of 3-MX and is shown to be capable of separating species with overlapping mass-to-charge ratios. FAIMS selected transmission also results in an improvement in signal-to-noise ratio for low intensity complexes and enables the visualisation of species undetectable without FAIMS.

History

School

  • Science

Department

  • Chemistry

Published in

Journal of the American Society for Mass Spectrometry

Citation

ARTHUR, K.L. ...et al., 2016. Analysis of supramolecular complexes of 3-methylxanthine with field asymmetric waveform ion mobility spectrometry combined with mass spectrometry. Journal of the American Society for Mass Spectrometry, 27 (5), pp. 800-809.

Publisher

© American Society for Mass Spectrometry. Published by Springer Verlag (Germany)

Version

AM (Accepted Manuscript)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2016

Notes

The final publication is available at Springer via http://dx.doi.org/10.1007/s13361-016-1351-y

ISSN

1044-0305

Language

en