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Anticalcification potential of heparin on hydroxyapatite seeds using a constant composition in vitro model

journal contribution
posted on 01.03.2019, 10:04 by Adel Badria, Petros Koutsoukos, Cristian D'Alessandro, Sotiris KorossisSotiris Korossis, Dimosthenis Mavrilas
Calcification is among the principal causes of biological heart valve substitute failure. Glycosaminoglcans (GAGs) are negatively charged molecules, possessing anticoagulation and anti-inflammatory activity that make them a potential solution against calcification. In the present work, the anticalcification potential of heparin was investigated under constant supersaturation conditions with respect to hydroxyapatite (Ca5(PO4)3OH; HAP). Heparin concentration in the supersaturated solutions was in the range between 0.25 and 3 ppm, at pH 7.40 and 37 °C. Heparin showed inhibitory activity, which was attributed to adsorption at the active crystal growth centres. Heparin concentration as low as ca. 50 μM, reduced the rate of HAP crystal growth by more than 80%, while further increase (up to 200 μM) failed to completely inhibit the process beyond 90%. Heparin uptake studies at equilibrium conditions and analysis of the kinetics data in the presence of heparin, strongly suggest that the inhibition is due to the adsorption of heparin onto the HAP crystals, which resulted in the poisoning of the active growth sites.

Funding

This research was supported by the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Program FP7/ 2007-2013/ under REA grant agreement n° 317512.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Journal of Crystal Growth

Volume

498

Pages

399 - 404

Citation

BADRIA, A. ... et al., 2018. Anticalcification potential of heparin on hydroxyapatite seeds using a constant composition in vitro model. Journal of Crystal Growth, 498, pp. 399 - 404.

Publisher

© Elsevier BV

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Acceptance date

10/07/2018

Publication date

2018-07-11

Notes

This paper is in closed access.

ISSN

0022-0248

Language

en

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