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Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells
journal contribution
posted on 05.03.2015, 16:15 authored by Andrea Ernst, Alexandra StolzingAlexandra Stolzing, Grit Sandig, Tilman GruneOxidative stress is supposed to play an important role in demyelinating diseases. Oligodendrocytes are the myelin-forming cells in the brain and are highly susceptible to oxidative stress due to their low antioxidative defense systems and high metabolic rate. In the present work, we tested the response of the oligodendrocyte cell line OLN 93 to oxidative stress. OLN 93 cell cultures are characterized by a loss of cell viability after oxidation. This loss of cell viability is accompanied by an increase in protein oxidation and consequently an elevated overall proteolysis. To minimize the oxidative damage, we tested the effects of the antioxidants α-lipoic acid and coenzyme Q10. Both compounds were able to elevate cell viability and to decrease intracellular protein turnover and oxidant induced protein oxidation. Therefore, we concluded that the excessive oxidative damage of oligodendrocytes and their protein pool can be prevented by the usage of antioxidants.
Funding
Deutsche Forschungsgemeinschaft and the Charite Research Funds
History
School
- Mechanical, Electrical and Manufacturing Engineering