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Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells

journal contribution
posted on 05.03.2015, 16:15 by Andrea Ernst, Alexandra StolzingAlexandra Stolzing, Grit Sandig, Tilman Grune
Oxidative stress is supposed to play an important role in demyelinating diseases. Oligodendrocytes are the myelin-forming cells in the brain and are highly susceptible to oxidative stress due to their low antioxidative defense systems and high metabolic rate. In the present work, we tested the response of the oligodendrocyte cell line OLN 93 to oxidative stress. OLN 93 cell cultures are characterized by a loss of cell viability after oxidation. This loss of cell viability is accompanied by an increase in protein oxidation and consequently an elevated overall proteolysis. To minimize the oxidative damage, we tested the effects of the antioxidants α-lipoic acid and coenzyme Q10. Both compounds were able to elevate cell viability and to decrease intracellular protein turnover and oxidant induced protein oxidation. Therefore, we concluded that the excessive oxidative damage of oligodendrocytes and their protein pool can be prevented by the usage of antioxidants.

Funding

Deutsche Forschungsgemeinschaft and the Charite Research Funds

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Archives of Biochemistry and Biophysics

Volume

421

Issue

1

Pages

54 - 60

Citation

ERNST, A. ... et al., 2004. Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells. Archives of Biochemistry and Biophysics, 421 (1), pp. 54 - 60.

Publisher

© Elsevier Inc

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2004

Notes

Closed access

ISSN

0003-9861

Language

en