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Automated, scalable culture of human embryonic stem cells in feeder free conditions
journal contributionposted on 17.02.2009 by Rob Thomas, David Anderson, Amit Chandra, Nigel M. Smith, Lorraine E. Young, David Williams, Chris Denning
Any type of content formally published in an academic journal, usually following a peer-review process.
Large-scale manufacture of human embryonic stem cells (hESCs) is prerequisite to their widespread use in biomedical applications. However, current hESC culture strategies are labor-intensive and employ highly variable processes, presenting challenges for scaled production and commercial development. Here we demonstrate that passaging of the hESC lines, HUES7, and NOTT1, with trypsin in feeder-free conditions, is compatible with complete automation on the CompacT SelecT, a commercially available and industrially relevant robotic platform. Pluripotency was successfully retained, as evidenced by consistent proliferation during serial passage, expression of stem cell markers (OCT4, NANOG, TRA1-81, and SSEA-4), stable karyotype, and multi-germlayer differentiation in vitro, including to pharmacologically responsive cardiomyocytes. Automation of hESC culture will expedite cell-use in clinical, scientific, and industrial applications.
- Mechanical, Electrical and Manufacturing Engineering