File(s) under permanent embargo

Reason: This item is currently closed access.

Automated, scalable culture of human embryonic stem cells in feeder free conditions

journal contribution
posted on 17.02.2009, 16:49 by Rob ThomasRob Thomas, David Anderson, Amit Chandra, Nigel M. Smith, Lorraine E. Young, David Williams, Chris Denning
Large-scale manufacture of human embryonic stem cells (hESCs) is prerequisite to their widespread use in biomedical applications. However, current hESC culture strategies are labor-intensive and employ highly variable processes, presenting challenges for scaled production and commercial development. Here we demonstrate that passaging of the hESC lines, HUES7, and NOTT1, with trypsin in feeder-free conditions, is compatible with complete automation on the CompacT SelecT, a commercially available and industrially relevant robotic platform. Pluripotency was successfully retained, as evidenced by consistent proliferation during serial passage, expression of stem cell markers (OCT4, NANOG, TRA1-81, and SSEA-4), stable karyotype, and multi-germlayer differentiation in vitro, including to pharmacologically responsive cardiomyocytes. Automation of hESC culture will expedite cell-use in clinical, scientific, and industrial applications.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Citation

THOMAS, R.J. ... et al, 2009. Automated, scalable culture of human embryonic stem cells in feeder-free conditions. Biotechnology and Bioengineering, 102 (6), pp. 1636-1644

Publisher

© Wiley Periodicals

Version

NA (Not Applicable or Unknown)

Publication date

2009

Notes

This article is Restricted Access. It was published in the journal, Biotechnology and Bioengineering [© Wiley] and is available at: http://www3.interscience.wiley.com/journal/117933915/grouphome/home.html

ISSN

0006-3592

Language

en