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P 2022 Biocompatibility of 3D-printed PLA, PEEK and PETG - Adhesion of bone marrow and peritoneal lavage cells.pdf (3.61 MB)

Biocompatibility of 3D-printed PLA, PEEK and PETG: Adhesion of bone marrow and peritoneal lavage cells

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posted on 2022-10-14, 11:01 authored by Stanislav Y Shilov, Yulia A Rozhkova, Lubov N Markova, Mikhail A Tashkinov, Ilya V Vindokurov, Vadim SilberschmidtVadim Silberschmidt
Samples in the form of cylindrical plates, additively manufactured using the fused deposition modelling (or filament freeform fabrication, FDM/FFF) technology from polylactide (PLA), polyethylene terephthalate glycol (PETG) and polyetheretherketone (PEEK), were studied in series of in-vitro experiments on the adhesion of rat bone-marrow cells and rat peritoneal cells. Methods of estimation of the absolute number of cells and polymer samples’ mass change were used for the evaluation of cells adhesion, followed by the evaluation of cell-culture supernatants. The results of experiments for both types of cells demonstrated a statistically significant change in the absolute number of cells (variation from 44 to 119%) and the weight of the polymer samples (variation from 0.61 to 2.18%), depending on roughness of sample surface, controlled by a nozzle diameter of a 3D printer as well as printing layer height. It was found that more cells adhere to PLA samples with a larger nozzle diameter and layer height. For PETG samples, the results did not show a clear relationship between cell adhesion and printing parameters. For PEEK samples, on the contrary, adhesion to samples printed with a lower nozzle diameter (higher resolution) is better than to samples printed with a larger nozzle diameter (lower resolution). The difference in results for various polymers can be explained by their chemical structure.

Funding

Perm National Research Polytechnic University

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Polymers

Volume

14

Issue

19

Publisher

MDPI

Version

  • VoR (Version of Record)

Rights holder

© The Authors

Publisher statement

This article is an Open Access article published by MDPI and distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/4.0/).

Acceptance date

2022-09-17

Publication date

2022-09-22

Copyright date

2022

eISSN

2073-4360

Language

  • en

Depositor

Prof Vadim Silberschmidt. Deposit date: 13 October 2022

Article number

3958

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