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Bright mono-aqua europium complexes based on triazacyclononane that bind anions reversibly and permeate cells efficiently

journal contribution
posted on 15.09.2015, 15:38 authored by Stephen ButlerStephen Butler, Brian K. McMahon, Robert Pal, David Parker, James W. Walton
A series of five europium(III) complexes has been prepared from heptadentate N5O2 ligands that possess a brightness of more than 10 mM−1 cm−1 in water, following excitation over the range λ=330–355 nm. Binding of several oxy anions has been assessed by emission spectral titrimetric analysis, with the binding of simple carboxylates, lactate and citrate involving a common ligation mode following displacement of the coordinated water. Selectivity for bicarbonate allows the rapid determination of this anion in human serum, with Kd=37 mM (295 K). The complexes are internalised quickly into mammalian cells and exhibit a mitochondrial localisation at early time points, migrating after a few hours to reveal a predominant lysosomal distribution. Herein, we report the synthesis and complexation behaviour of strongly emissive europium (III) complexes that bind oxy-anions in aqueous media with an affinity and selectivity profile that is distinctively different from previously studied systems.

Funding

We thank the ERC (FCC 266804) and CISbio Bioassys (J.W.W.) for support.

History

School

  • Science

Department

  • Chemistry

Published in

CHEMISTRY-A EUROPEAN JOURNAL

Volume

19

Issue

29

Pages

9511 - 9517 (7)

Citation

BUTLER, S.J. ... et al, 2013. Bright mono-aqua europium complexes based on triazacyclononane that bind anions reversibly and permeate cells efficiently. Chemistry - A European Journal, 19 (29), pp. 9511 - 9517.

Publisher

© Wiley-VCH Verlag GmbH & Co.

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2013

Notes

This article is closed access.

ISSN

0947-6539

Language

en