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Characterisation of the protein corona using tunable resistive pulse sensing: determining the change and distribution of a particle’s surface charge
journal contribution
posted on 2016-06-17, 08:37 authored by Emma Blundell, Matthew Healey, Elizabeth Holton, Muttuswamy Sivakumaran, Sarabjit MastanaSarabjit Mastana, Mark PlattMark PlattThe zeta potential of the protein corona around carboxyl
particles has been measured using tunable resistive
pulse sensing (TRPS). A simple and rapid assay for characterising
zeta potentials within buffer, serum and plasma is presented
monitoring the change, magnitude and distribution of
proteins on the particle surface. First, we measure the change
in zeta potential of carboxyl-functionalised nanoparticles in
solutions that contain biologically relevant concentrations of
individual proteins, typically constituted in plasma and serum,
and observe a significant difference in distributions and zeta
values between room temperature and 37 °C assays. The effect
is protein dependent, and the largest difference between the
two temperatures is recorded for the γ-globulin protein where
the mean zeta potential changes from −16.7 to −9.0 mV for 25
and 37 °C, respectively. This method is further applied to
monitor particles placed into serum and/or plasma. A
temperature-dependent change is again observed with serum
showing a 4.9 mV difference in zeta potential between samples
incubated at 25 and 37 °C; this shift was larger than that
observed for samples in plasma (0.4 mV). Finally, we monitor
the kinetics of the corona reorientation for particles initially placed into serum and then adding 5 % (V/V) plasma. The
technology presented offers an interesting insight into protein
corona structure and kinetics of formation measured in biologically
relevant solutions, i.e. high protein, high salt levels,
and its particle-by-particle analysis gives a measure of the
distribution of particle zeta potential that may offer a better
understanding of the behaviour of nanoparticles in solution.
Funding
The work was supported by the European Commission for Research (PCIG11-GA-2012-321836 Nano4Bio) and the Peterborough City Hospital Haematology Research Fund. E.L.C.J.B. is supported by Izon Science Ltd and Loughborough University. EH was funded by the Analytical Science Trust fund for a summer studentship.
History
School
- Science
Department
- Chemistry
Published in
Analytical and Bioanalytical ChemistryCitation
BLUNDELL, E. ... et al, 2016. Characterisation of the protein corona using tunable resistive pulse sensing: determining the change and distribution of a particle’s surface charge. Analytical and Bioanalytical Chemistry, 408 (21), pp. 5757-5768.Publisher
Springer Verlag / © The AuthorsVersion
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/ by/4.0/Publication date
2016Notes
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http:/ / creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.ISSN
1618-2650Publisher version
Language
- en