Comparability of automated human induced pluripotent stem cell culture: a pilot study
journal contributionposted on 01.09.2016 by Peter R.T. Archibald, Amit Chandra, Dave Thomas, Olivier Chose, Emmanuelle Massourides, Yacine Laabi, David Williams
Any type of content formally published in an academic journal, usually following a peer-review process.
Consistent and robust manufacturing is essential for the translation of cell therapies, and the utilisation automation throughout the manufacturing process may allow for improvements in quality control, scalability, reproducibility and economics of the process. The aim of this study was to measure and establish the comparability between alternative process steps for the culture of hiPSCs. Consequently, the effects of manual centrifugation and automated non-centrifugation process steps, performed using TAP Biosystems’ CompacT SelecT automated cell culture platform, upon the culture of a human induced pluripotent stem cell (hiPSC) line (VAX001024c07) were compared. This study, has demonstrated that comparable morphologies and cell diameters were observed in hiPSCs cultured using either manual or automated process steps. However, non-centrifugation hiPSC populations exhibited greater cell yields, greater aggregate rates, increased pluripotency marker expression, and decreased differentiation marker expression compared to centrifugation hiPSCs. A trend for decreased variability in cell yield was also observed after the utilisation of the automated process step. This study also highlights the detrimental effect of the cryopreservation and thawing processes upon the growth and characteristics of hiPSC cultures, and demonstrates that automated hiPSC manufacturing protocols can be successfully transferred between independent laboratories.
Funding and support of the EPSRC for the EPSRC Centre for Innovative Manufacturing in Regenerative Medicine is acknowledged.
- Mechanical, Electrical and Manufacturing Engineering