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Comparability of scalable, automated hMSC culture using manual and automated process steps

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posted on 20.08.2015 by Peter Archibald, Amit Chandra, Dave Thomas, Gary Morley, Tamara Lekishvili, Alison Devonshire, David Williams
Automation will likely to play a key role in the development of scalable manufacturing processes for cell-based therapies. In this study, we have compared the effects of manual centrifugation and automated non-centrifugation cell culture process steps, performed using TAP biosystems’ CompacT SelecT automated cell culture platform, upon hMSC morphology, number, viability, surface marker expression, Short tandem repeat (STR) profile, and paracrine function. Furthermore, the comparability between flow cytometry analyses of hMSCs, performed at multiple sites, was investigated. No significant difference in hMSC growth and characteristics was observed between cells cultured using either the manual centrifugation process step or the automated non-centrifugation process step, in which residual dissociation agent is carried over. However, some variability in paracrine activity was observed between hMSCs cultured using alternative process steps. It is also apparent that differences in analytical methods can influence the inter-laboratory reproducibility of hMSC flow cytometry analysis, although differences in culture may also contribute to the variability observed in the expression of 2 of the 8 surface markers examined. This novel investigation into the effects of these two key process steps will help to improve the understanding of the influence of automated cell culture upon various cell culture parameters, as well as upon process comparability.

Funding

TAP Biosystems, Loughborough University and the Engineering and Physical Sciences Research Council (EPSRC) [grant number: EP/H028277/1].

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Biochemical Engineering Journal

Citation

ARCHIBALD, P.R.T. et al., 2016. Comparability of scalable, automated hMSC culture using manual and automated process steps. Biochemical Engineering Journal, 108, pp.69-83.

Publisher

© The Authors. Published by Elsevier B.V

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/ by/4.0/

Publication date

2016

Notes

This is an open access article published by Elsevier under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

ISSN

1873-295X

Language

en

Location

United Kingdom

Licence

Exports