Continuous protein crystallisation platform and process: Case of lysozyme
journal contributionposted on 2019-07-01, 13:08 authored by Huaiyu YangHuaiyu Yang, Peter Peczulis, Pavan Inguva, Xiaoyu Li, Jerry Y.Y. Heng
© 2018 Institution of Chemical Engineers In this work, we designed and built a continuous crystallisation oscillatory flow platform. The lysozyme crystallisation behaviours were investigated at concentrations from 30 to 100 mg/mL, under oscillatory conditions with amplitude (x0) from 10 to 25 mm and frequency (f) from 0.05 to 0.25 Hz in a batch oscillatory flow crystallisation platform. The nucleation rate increased with increase in concentration of initial lysozyme solution, and was also found to increase with increase in shear rate. By learning the thermodynamics and kinetics of lysozyme crystallisation in batch oscillatory flow, the batch crystallisation process was successfully transferred to a continuous oscillatory flow crystallisation process. The equilibrium state of continuous crystallisation reached at residence time 200 min, and the final product crystals shape and size were consistent during the continuous process. This work demonstrates the feasibility of oscillatory flow based platforms for the development of continuous protein crystallisation as for downstream bioseparation.
We acknowledge the EPSRC (EP/N015916/1) for funding.
- Aeronautical, Automotive, Chemical and Materials Engineering
- Chemical Engineering
Published inChemical Engineering Research and Design
Pages529 - 535
CitationYANG, H. ... et al., 2018. Continuous protein crystallisation platform and process: Case of lysozyme. Chemical Engineering Research and Design, 136, pp. 529 - 535.
Publisher© Institution of Chemical Engineers. Published by Elsevier
- AM (Accepted Manuscript)
Publisher statementThis work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/
NotesThis paper was accepted for publication in the journal Chemical Engineering Research and Design and the definitive published version is available at https://doi.org/10.1016/j.cherd.2018.05.031