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Creatine and creatinine quantification in Olympic athletes: dried blood spot analysis pilot study

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posted on 2022-11-17, 09:12 authored by Flavio I Bachini, Danilo Pereira, Ruan Santos, Matheus Hausen, Glauber Pereira, Camila Vieira, Lee TaylorLee Taylor, Marcelle Pegurier

Capillary dried blood spot (DBS) samples facilitate field-based collection without venipuncture. This pilot study aims to evaluate the viability of creatine (Cr) and creatinine (Crt) quantification using fresh capillary serum (CrS/CrtS) and DBS samples (CrDBS/CrtDBS), using Flow Injection Analysis Mass Spectrometry (FIA – MS). Nine Olympic Athletes provided a capillary blood sample to assess CrS/CrtS and CrDBS/CrtDBS quantified by FIA – MS. No difference between CrtS (mean ± SD: 813.6 ± 102.4 µmol/L) and CrtDBS (812.4 ± 108.1 µmol/L) was observed with acceptable variance [SEM 88.7; CV 10.7%; ICC 0.57 (CI 95% 0.06 – 0.84)] and agreement [very strong (Spearman: r = 0.77; p < 0.01) or strong (Pearson: r = 0.56; p = 0.04); Bland Altman: lower (-193) and upper (+196) limits of agreement]. CrS (mean ± SD: 691.8 ± 165.2 µmol/L) was significantly different to CrDBS (2911 ± 571.4 µmol/L) with unacceptable variance [SEM 171.6; CV 27%; ICC 0.002 (CI 95% -0.02 – 0.07)] and ‘weak’ agreement [Spearman: r = 0.21, p = 0.47 and Pearson: r = 0.06, p = 0.84; Bland Altman lower (-3367) and upper (-1072) limits of agreement]. Crt quantification is viable using both CrtS and CrtDBS (but not for Cr and CrS/CrDBS), with the DBS tissue handling technique offering several methodological and practice facing advantages. Future work should expand upon the sample size, explore sport/discipline relevant analytes across a full competitive season, including key training, recovery and performance blocks of their periodized performance plan.

History

School

  • Sport, Exercise and Health Sciences

Published in

Biology of Sport

Volume

39

Issue

3

Pages

745 - 749

Publisher

Termedia Publishing House

Version

  • VoR (Version of Record)

Rights holder

© Institute of Sport – National Research Institute in Warsaw, Poland

Publisher statement

This is an Open Access Article. It is published by Termedia Publishing House under the Creative Commons Attribution-ShareAlike 4.0 International Licence (CC BY-SA). Full details of this licence are available at: https://creativecommons.org/licenses/by-sa/4.0/

Acceptance date

2021-07-16

Publication date

2021-09-30

Copyright date

2021

ISSN

0860-021X

eISSN

2083-1862

Language

  • en

Depositor

Deposit date: 16 November 2022

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