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Culture on fibrin matrices maintains the colony-forming capacity and osteoblastic differentiation of mesenchymal stem cells
journal contribution
posted on 2015-02-26, 15:28 authored by Helen Colley, Sally L. McArthur, Alexandra StolzingAlexandra Stolzing, Andrew ScuttMesenchymal stem cells (MSC) are multipotent cells capable of differentiating into a number of mesenchymal tissues including bone, cartilage, and tendon. Low numbers in vivo means exponential growth is needed in culture to enable therapeutic applications. MSC can expand rapidly in culture but usually lose their extensive capacity for differentiation that makes them therapeutically attractive. To try and maintain their capacity for differentiation and expansion in vitro, we cultured MSC on fibrin gels of different concentrations to create more physiological growth conditions for the cells. The cells were then re-plated onto tissue culture plastic and analysed. The cells that had been pre-cultured for seven days on fibrin, proliferated and maintained their differential potential to the osteogenic lineage better than tissue culture plastic expanded MSC. A concentration relationship between colony number and fibrin concentration was seen with decreasing numbers as fibrin concentration increased. These data support the concept that substrate signals significantly influence MSC growth and differentiation and that growth on a fibrin matrix could be used to maintain a stem cell phenotype during MSC expansion. © 2012 IOP Publishing Ltd.
Funding
HEC was in receipt of a Departmental Training Allocation studentship from the EPSRC and ASt was funded by the BBSRC. ASc and SLM were HEFCE funded permanent members of staff.
History
School
- Mechanical, Electrical and Manufacturing Engineering
Published in
Biomedical Materials (Bristol)Volume
7Issue
4Citation
COLLEY, H. ... et al, 2012. Culture on fibrin matrices maintains the colony-forming capacity and osteoblastic differentiation of mesenchymal stem cells. Biomedical Materials, 7 (4), 045015.Publisher
© Institute of Physics PublishingVersion
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2012Notes
This article is closed access.ISSN
1748-6041eISSN
1748-605XPublisher version
Language
- en