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DROPLAY: laser writing of functional patterns within biological microdroplet displays

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posted on 15.12.2016, 11:41 by Chi Long Chan, Guido BolognesiGuido Bolognesi, Archis Bhandarkar, Mark S. Friddin, Nicholas J. Brooks, John M. Seddon, Robert V. Law, Laura M.C. Barter, Oscar Ces
In this study, we introduce an optofluidic method for the rapid construction of large-area cell-sized droplet assemblies with user-defined, re-writable, two-dimensional patterns of functional droplets. Light responsive water-in-oil droplets capable of releasing fluorescent dye molecules upon exposure were generated and self-assembled into arrays inside a microfluidic device. This biological architecture was exploited by the scanning laser of a confocal microscope to ‘write’ user defined patterns of differentiated (fluorescent) droplets in a network of originally undifferentiated (non-fluorescent) droplets. As a result, long lasting images were produced on a droplet fabric with droplets acting as pixels of a biological monitor, which can be erased and re-written on-demand. Regio-specific light-induced droplet differentiation within a large population of droplets provides a new paradigm for the rapid construction of bio-synthetic systems with potential as tissue mimics and biological display materials.

Funding

This research was funded by EP/J017566/1, EP/L015498/1 and EP/K503733/1 grants.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Lab on a Chip - Miniaturisation for Chemistry and Biology

Volume

16

Issue

23

Pages

4621 - 4627

Citation

CHAN, C.L. ... et al, 2016. DROPLAY: laser writing of functional patterns within biological microdroplet displays. Lab on a Chip, 16 (23), pp. 4621 - 4627

Publisher

Royal Society of Chemistry

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/

Publication date

2016

Notes

This is an open access article published by the Royal Society of Chemistry and distributed under the terms of the Creative Commons Attribution Licence (CC BY 3.0), https://creativecommons.org/licenses/by/3.0/

ISSN

1473-0197

eISSN

1473-0189

Language

en

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