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Design and validation of a physiologically-adapted bioreactor for tissue engineering of the nucleus pulposus

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posted on 30.01.2014, 09:10 authored by May Win Naing, Yang LiuYang Liu, Immanuel Sebastine, David Dingmann, Chrysanthi Williams, David Williams
A novel multi-axial bioreactor was designed and developed to deliver combinations of the following dynamic mechanical stimulation conditions: hydrostatic pressure, pulsatile perfusion flow and uniaxial compression in order to mimic in vivo conditions. This mechanical arrangement simultaneously allows triaxial stimulation and characterization of mechanical properties of samples, in particular simulating the conditions experienced by the nucleus pulposus in vivo. A series of initial experiments were performed on this prototype system using consistent, commercially-available, three dimensional scaffolds in combination with human dermal fibroblasts. Our results show that while such bioreactors hold much promise in tissue engineering of desired organs, achieving the right combination of mechanical stimuli and other conditions required in order to enhance the final properties of the cell-scaffold systems is challenging.

Funding

This work was supported by the Engineering and Physical Sciences Research Council (EPSRC) [grant number EP/C534247/1].

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Citation

WIN NAING, M. ... et al., 2013. Design and validation of a physiologically-adapted bioreactor for tissue engineering of the nucleus pulposus. Processes, 2 (1), pp. 1-11.

Publisher

© 2013 by the authors; licensee MDPI, Basel, Switzerland

Version

VoR (Version of Record)

Publication date

2013

Notes

This is an Open Access Article. It is published by MDPI, Basel, Switzerland under the Creative Commons Attribution 3.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/

eISSN

2227-9717

Language

en