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Design modification and optimisation of the perfusion system of a tri-axial bioreactor for tissue engineering

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posted on 2015-03-19, 15:12 authored by Husnah Hussein, David Williams, Yang LiuYang Liu
A systematic design of experiments (DOE) approach was used to optimize the perfusion process of a tri-axial bioreactor designed for translational tissue engineering exploiting mechanical stimuli and mechanotransduction. Four controllable design parameters affecting the perfusion process were identified in a cause–effect diagram as potential improvement opportunities. A screening process was used to separate out the factors that have the largest impact from the insignificant ones. DOE was employed to find the settings of the platen design, return tubing configuration and the elevation difference that minimise the load on the pump and variation in the perfusion process and improve the controllability of the perfusion pressures within the prescribed limits. DOE was very effective for gaining increased knowledge of the perfusion process and optimizing the process for improved functionality. It is hypothesized that the optimized perfusion system will result in improved biological performance and consistency.


This work has been partially supported by the EPSRC (Engineering and Physical Sciences Research Council UK) Centre for Innovative Manufacturing in Regenerative Medicine, Royal Society International Joint Projects (JP101627) and FP7- PEOPLE-2012- IRSES (SkelGen). H. Hussein is supported by the EPSRC Regenerative Medicine Doctoral Training Centre (DTC).



  • Mechanical, Electrical and Manufacturing Engineering

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Bioprocess and Biosystems Engineering


HUSSEIN, H., WILLIAMS, D.J. and LIU, Y., 2015. Design modification and optimisation of the perfusion system of a tri-axial bioreactor for tissue engineering. Bioprocess and Biosystems Engineering. 38(7), pp.1423-1429.


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