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Design modification and optimisation of the perfusion system of a tri-axial bioreactor for tissue engineering

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journal contribution
posted on 19.03.2015, 15:12 by Husnah Hussein, David Williams, Yang LiuYang Liu
A systematic design of experiments (DOE) approach was used to optimize the perfusion process of a tri-axial bioreactor designed for translational tissue engineering exploiting mechanical stimuli and mechanotransduction. Four controllable design parameters affecting the perfusion process were identified in a cause–effect diagram as potential improvement opportunities. A screening process was used to separate out the factors that have the largest impact from the insignificant ones. DOE was employed to find the settings of the platen design, return tubing configuration and the elevation difference that minimise the load on the pump and variation in the perfusion process and improve the controllability of the perfusion pressures within the prescribed limits. DOE was very effective for gaining increased knowledge of the perfusion process and optimizing the process for improved functionality. It is hypothesized that the optimized perfusion system will result in improved biological performance and consistency.

Funding

This work has been partially supported by the EPSRC (Engineering and Physical Sciences Research Council UK) Centre for Innovative Manufacturing in Regenerative Medicine, Royal Society International Joint Projects (JP101627) and FP7- PEOPLE-2012- IRSES (SkelGen). H. Hussein is supported by the EPSRC Regenerative Medicine Doctoral Training Centre (DTC).

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Bioprocess and Biosystems Engineering

Citation

HUSSEIN, H., WILLIAMS, D.J. and LIU, Y., 2015. Design modification and optimisation of the perfusion system of a tri-axial bioreactor for tissue engineering. Bioprocess and Biosystems Engineering. 38(7), pp.1423-1429.

Publisher

Springer Berlin Heidelberg (© The Authors)

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/

Publication date

2015

Notes

This article is published with open access at Springerlink.com. This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

ISSN

1615-7591

eISSN

1615-7605

Language

en

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