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Determination of free desmosine and isodesmosine as urinary biomarkers of lung disorder by ultra performance liquid chromatography-ion mobility-mass spectrometry

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journal contribution
posted on 26.10.2011, 12:49 by Neil A. Devenport, Jim ReynoldsJim Reynolds, Ved Parkash, Jason Cook, Daniel J. Weston, Colin Creaser
The elastin degradation products, desmosine (DES) and isodesmosine (IDES) are highly stable, cross-linking amino-acids that are unique to mature elastin. The excretion of DES/IDES in urine, in the free form and with associated peptide fragments, provides an indicator of lung damage in chronic obstructive pulmonary disease (COPD). A quantitative ion mobility-mass spectrometry (IM-MS) method has been developed for the analysis of free DES/IDES in urine with deuterated IDES as an internal standard. Resolution of DES/IDES isomers was achieved in less than five minutes using ultra performance liquid chromatography (UPLC) combined with ion pairing. The optimized UPLC-IM-MS method provided a linear dynamic range of 10-300 ng/mL and a limit of quantitation of 0.028 ng/mL for IDES and 0.03 ng/mL for DES (0.55 ng and 0.61 ng on column respectively). The method reproducibility (%RSD) was < 4% for DES and IDES. The UPLC-IM-MS method was applied to the analysis of urine samples obtained from healthy volunteers and COPD patients. The DES/IDES concentrations in healthy and COPD urine showed an increase in DES (79%) and IDES (74%) in the COPD samples, relative to healthy controls. The incorporation of an IM separation prior to m/z measurement by MS was shown to reduce non-target ion responses from the bio-fluid matrix.

History

School

  • Science

Department

  • Chemistry

Citation

DEVENPORT, N.A. ... et al, 2011. Determination of free desmosine and isodesmosine as urinary biomarkers of lung disorder by ultra performance liquid chromatography-ion mobility-mass spectrometry. Journal of Chromatography B, 879 (32), pp. 3797-3801

Publisher

© Elsevier

Version

AM (Accepted Manuscript)

Publication date

2011

Notes

This article was published in the serial, Journal of Chromatography B [© Elsevier]. The definitive version is available at: http://www.sciencedirect.com/science/article/pii/S1570023211006817

ISSN

1570-0232

Language

en