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Determination of inflammatory and prominent proteomic changes in plasma and adipose tissue after high intensity intermittent training in overweight and obese males

journal contribution
posted on 20.04.2012, 08:51 by Melanie Leggate, Wayne Carter, Matthew J.C. Evans, Rebecca A. Vennard, Sarah Sribala-Sundaram, Myra A. Nimmo
This study aimed to determine whether 2 wk of high-intensity intermittent training (HIIT) altered inflammatory status in plasma and adipose tissue in overweight and obese males. Twelve participants [mean (SD): age 23.7 (5.2) yr, body mass 91.0 (8.0) kg, body mass index 29.1 (3.1) kg/m2] undertook six HIIT sessions over 2 wk. Resting blood and subcutaneous abdominal adipose tissue samples were collected and insulin sensitivity determined, pre- and posttraining. Inflammatory proteins were quantified in plasma and adipose tissue. There was a significant decrease in soluble interleukin-6 receptor (sIL-6R; P = 0.050), monocyte chemotactic protein-1 (MCP-1, P = 0.047), and adiponectin (P = 0.041) in plasma posttraining. Plasma IL-6, intercellular adhesion molecule-1 (ICAM-1), tumor necrosis factor-α (TNF-α), IL-10, and insulin sensitivity did not change. In adipose tissue, IL-6 significantly decreased (P = 0.036) and IL-6R increased (P = 0.037), while adiponectin tended to decrease (P = 0.056), with no change in ICAM-1 posttraining. TNF-α, MCP-1, and IL-10 were not detectable in adipose tissue. Adipose tissue homogenates were then resolved using one-dimensional gel electrophoresis, and major changes in the adipose tissue proteome, as a consequence of HIIT, were evaluated. This proteomic approach identified significant reductions in annexin A2 (P = 0.046) and fatty acid synthase (P = 0.016) as a response to HIIT. The present investigation suggests 2 wk of HIIT is sufficient to induce beneficial alterations in the resting inflammatory profile and adipose tissue proteome of an overweight and obese male cohort.

History

School

  • Sport, Exercise and Health Sciences

Citation

LEGGATE, M. ... et al., 2012. Determination of inflammatory and prominent proteomic changes in plasma and adipose tissue after high intensity intermittent training in overweight and obese males. Journal of Applied Physiology, 112 (8), pp. 1353 - 1360

Publisher

© American Physiological Society

Version

AM (Accepted Manuscript)

Publication date

2012

Notes

This article is closed access. It was published in the Journal of Applied Physiology [© the American Physiological Society] and the definitive version is available at: http://dx.doi.org/10.​1152/​japplphysiol.​01080.​2011

ISSN

0021-8987

Language

en

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