Brown et al., 2019.pdf (1 MB)
Determining conditions for successful culture of multi-cellular 3D tumour spheroids to investigate the effect of mesenchymal stem cells on breast cancer cell invasiveness
journal contribution
posted on 2019-11-04, 11:20 authored by Marie-Juliet Brown, Soukaina Bahsoun, Mhairi MorrisMhairi Morris, Liz AkamLiz AkamMesenchymal stem cells have been widely implicated in tumour development and
metastases. Moving from the use of two-dimensional (2D) models to three-dimensional (3D) to
investigate this relationship is critical to facilitate more applicable and relevant research on the tumour
microenvironment. We investigated the effects of altering glucose concentration and the source of
foetal bovine serum (FBS) on the growth of two breast cancer cell lines (T47D and MDA-MB-231) and
human bone marrow-derived mesenchymal stem cells (hBM-MSCs) to determine successful
conditions to enable their co-culture in 3D tumour spheroid models. Subsequently, these 3D multicellular tumour spheroids were used to investigate the effect of hBM-MSCs on breast cancer cell
invasiveness. Findings presented herein show that serum source had a statistically significant effect
on two thirds of the growth parameters measured across all three cell lines, whereas glucose only had
a statistically significant effect on 6%. It was determined that the optimum growth media composition
for the co-culture of 3D hBM-MSCs and breast cancer cell line spheroids was 1 g/L glucose DMEM
supplemented with 10% FBS from source A. Subsequent results demonstrated that co-culture of hBMMSCs and MDA-MB-231 cells dramatically reduced invasiveness of both cell lines (F(1,4) = 71.465, p =
0.001) when embedded into a matrix comprising of growth-factor reduced base membrane extract
(BME) and collagen.
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School
- Sport, Exercise and Health Sciences
Published in
BioengineeringVolume
6Issue
4Publisher
MDPIVersion
- VoR (Version of Record)
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© The AuthorsPublisher statement
This is an Open Access Article. It is published by MDPI under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/Acceptance date
2019-10-30Publication date
2019-11-01Copyright date
2019ISSN
2306-5354Publisher version
Language
- en
Depositor
Dr Mhairi Morris Deposit date: 1 November 2019Article number
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