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Development and characterization of a porcine mitral valve scaffold for tissue engineering
journal contributionposted on 2019-03-01, 11:17 authored by M. Granados, Lucrezia Morticelli, S. Andriopoulou, P. Kalozoumis, Michael Pflaum, P. Iablonskii, B. Glasmacher, M. Harder, J. Hegermann, C. Wrede, I. Tudorache, S. Cebotari, Andres Hilfiker, Axel Haverich, Sotiris KorossisSotiris Korossis
Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% (w/ v) SDS and 0.5% (w/v) SD and sterilized with 0.1% (v/v) PAA. Decellularized samples were seeded with human foreskin fibroblasts and human adipose-derived stem cells to investigate cellular repopulation and infiltration, and with human colonyforming endothelial cells to investigate collagen IV formation. Histology revealed an acellular scaffold with a generally conserved histoarchitecture, but collagen IV loss. Following decellularization, no significant changes were observed in the hydroxyproline content, but there was a significant reduction in the glycosaminoglycan content. SEM/TEM analysis confirmed cellular removal and loss of some extracellular matrix components. Collagen and elastin were generally preserved. The endothelial cells produced newly formed collagen IVon the non-cytotoxic scaffold. The protocol produced acellular scaffolds with generally preserved histoarchitecture, biochemistry, and biomechanics.
This research was supported by the People Programme (Marie Curie Actions) of the EU 7th Framework Programme FP7/2007–2013/ under the REA grant agreement number 317512, and the German Research Foundation through the Cluster of Excellence REBIRTH (From Regenerative Biology to Reconstructive Therapy; EXC 62).
- Mechanical, Electrical and Manufacturing Engineering
Published inJournal of Cardiovascular Translational Research
Pages374 - 390
CitationGRANADOS, M. ... et al., 2017. Development and characterization of a porcine mitral valve scaffold for tissue engineering. Journal of Cardiovascular Translational Research, 10(4), pp. 374 - 390.
Publisher© Springer Nature
- VoR (Version of Record)
Publisher statementThis work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/
NotesThis paper is in closed access.
Mitral valveHeart valve replacementDecellularizationBiomechanicsHistologyImmunohistochemistryBiochemistryα-GalXenoepitopeCollagen IVBiocompatibilityTissue engineeringScaffoldTransmission electron microscopyScanning electron microscopyCytotoxicityScaffold seedingHuman foreskin fibroblastsHumanadipose-derived stem cellsHuman colony-forming endothelial cellsMechanical Engineering not elsewhere classified