LYS workflow final-accept.pdf (1.35 MB)
Development and workflow of a continuous protein crystallization process: A case of lysozyme
journal contribution
posted on 2019-04-25, 13:57 authored by Huaiyu YangHuaiyu Yang, Wenqian Chen, Peter Peczulis, Jerry Y.Y. HengIn the present work, a workflow on the development of a continuous protein crystallization is introduced, with lysozyme as a model protein, from microliter screening experiments, to small scale batch crystallization experiments in a shaking crystallization platform, and to batch and continuous crystallization experiments in an oscillatory flow platform. The lysozyme crystallizations investigated were for a concentration range from 30 to 100 mg/mL, shaking conditions from 100 to 200 rpm in the batch shaking crystallization platform, and oscillatory conditions with amplitude (x 0 ) from 5 to 30 mm and frequency (f) from 0.1 to 1.0 Hz in the batch oscillatory flow crystallization platform. We propose the use of the Reynold's number (R e ) for scaling up of the process from the shaking batch to the continuous oscillatory flow platform. Additionally, it is shown that the nucleation rate increased with increase in concentration of initial lysozyme solution, or increase in shear rate, inducing smaller size of lysozyme crystals. The properties and qualities of the crystal products indicate that continuous crystallization platforms may offer advantages to the downstream bioprocessing of proteins.
Funding
EPSRC (EP/N015916/1)
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Crystal Growth and DesignVolume
19Issue
2Pages
983 - 991Citation
YANG, H. ... et al, 2019. Development and workflow of a continuous protein crystallization process: A case of lysozyme. Crystal Growth and Design, 19 (2), pp.983-991.Publisher
© American Chemical SocietyVersion
- AM (Accepted Manuscript)
Publisher statement
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Crystal Growth and Design, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.cgd.8b01534.Publication date
2019-01-04ISSN
1528-7483eISSN
1528-7505Publisher version
Language
- en