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Download fileDevelopment of a novel 3D culture system for screening features of a complex implantable device for CNS repair
journal contribution
posted on 2017-05-12, 08:39 authored by Peter S. Donoghue, Tao SunTao Sun, Nikolaj Gadegaard, Mathis O. Riehle, Susan C. BarnettTubular scaffolds which incorporate a variety of micro- and
nanotopographies have a wide application potential in tissue engineering
especially for the repair of spinal cord injury (SCI). We aim to produce
metabolically active differentiated tissues within such tubes, as it is crucially
important to evaluate the biological performance of the three-dimensional (3D)
scaffold and optimize the bioprocesses for tissue culture. Because of the
complex 3D configuration and the presence of various topographies, it is rarely
possible to observe and analyze cells within such scaffolds in situ. Thus, we aim
to develop scaled down mini-chambers as simplified in vitro simulation systems,
to bridge the gap between two-dimensional (2D) cell cultures on structured
substrates and three-dimensional (3D) tissue culture. The mini-chambers were
manipulated to systematically simulate and evaluate the influences of gravity,
topography, fluid flow, and scaffold dimension on three exemplary cell models
that play a role in CNS repair (i.e., cortical astrocytes, fibroblasts, and myelinating cultures) within a tubular scaffold created by
rolling up a microstructured membrane. Since we use CNS myelinating cultures, we can confirm that the scaffold does not affect
neural cell differentiation. It was found that heterogeneous cell distribution within the tubular constructs was caused by a
combination of gravity, fluid flow, topography, and scaffold configuration, while cell survival was influenced by scaffold length,
porosity, and thickness. This research demonstrates that the mini-chambers represent a viable, novel, scale down approach for the
evaluation of complex 3D scaffolds as well as providing a microbioprocessing strategy for tissue engineering and the potential
repair of SCI.
Funding
We gratefully acknowledge the financial support from BBSRC (U.K.) (grant number: BBG0047061) for this study.
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Molecular PharmaceuticsVolume
11Issue
7Pages
2143 - 2150Citation
DONOGHUE, P.S. ... et al, 2014. Development of a novel 3D culture system for screening features of a complex implantable device for CNS repair. Molecular Pharmaceutics, 11 (7), pp.2143-2150Publisher
© American Chemical SocietyVersion
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Acceptance date
2013-11-25Publication date
2013-12-06Copyright date
2014Notes
This is an ACS AuthorChoice article.ISSN
1543-8384eISSN
1543-8392Publisher version
Language
- en