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Digitally driven aerosol jet printing to enable customisable neuronal guidance

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posted on 12.10.2021, 08:19 by Andrew CapelAndrew Capel, Matthew A. A. Smith, Silvia Taccola, Maria Pardo-Figuerez, Rowan RimingtonRowan Rimington, Mark LewisMark Lewis, Steven ChristieSteven Christie, Robert W. Kay, Russell A. Harris
Digitally driven manufacturing technologies such as aerosol jet printing (AJP) can make a significant contribution to enabling new capabilities in the field of tissue engineering disease modeling and drug screening. AJP is an emerging non-contact and mask-less printing process which has distinct advantages over other patterning technologies as it offers versatile, high-resolution, direct-write deposition of a variety of materials on planar and non-planar surfaces. This research demonstrates the ability of AJP to print digitally controlled patterns that influence neuronal guidance. These consist of patterned poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) tracks on both glass and poly(potassium 3-sulfopropyl methacrylate) (PKSPMA) coated glass surfaces, promoting selective adhesion of SH-SY5Y neuroblastoma cells. The cell attractive patterns had a maximum height ≥0.2 μm, width and half height ≥15 μm, Ra = 3.5 nm, and RMS = 4.1. The developed biocompatible PEDOT:PSS ink was shown to promote adhesion, growth and differentiation of SH-SY5Y neuronal cells. SH-SY5Y cells cultured directly onto these features exhibited increased nuclei and neuronal alignment on both substrates. In addition, the cell adhesion to the substrate was selective when cultured onto the PKSPMA surfaces resulting in a highly organized neural pattern. This demonstrated the ability to rapidly and flexibly realize intricate and accurate cell patterns by a computer controlled process.

Funding

Towards Bespoke Bio-Hybrid Prosthesis - Manufacturing bio-inductive interfaces in 3D

Engineering and Physical Sciences Research Council

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A Platform for Hybrid Manufacturing Process research

Engineering and Physical Sciences Research Council

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Towards Bespoke Bio-Hybrid Prosthesis - Manufacturing bio-inductive interfaces in 3D

Engineering and Physical Sciences Research Council

Find out more...

History

School

  • Science
  • Sport, Exercise and Health Sciences

Department

  • Chemistry

Published in

Frontiers in Cell and Developmental Biology

Volume

9

Publisher

Frontiers Media

Version

VoR (Version of Record)

Rights holder

© The authors

Publisher statement

This is an Open Access Article. It is published by Frontiers Media under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/

Acceptance date

04/08/2021

Publication date

2021-08-30

Copyright date

2021

ISSN

2296-634X

eISSN

2296-634X

Language

en

Depositor

Dr Andrew Capel. Deposit date: 11 October 2021

Article number

722294