Published.pdf (1.4 MB)
Direct manipulation of liquid ordered lipid membrane domains using optical traps
journal contribution
posted on 2019-02-11, 14:33 authored by Mark S. Friddin, Guido Bolognesi, Ali Salehi-Reyhani, Oscar Ces, Yuval ElaniMulticomponent lipid bilayers can give rise to coexisting liquid domains that are thought to
influence a host of cellular activities. There currently exists no method to directly manipulate
such domains, hampering our understanding of their significance. Here we report a system
that allows individual liquid ordered domains that exist in a liquid disordered matrix to be
directly manipulated using optical tweezers. This allows us to drag domains across the
membrane surface of giant vesicles that are adhered to a glass surface, enabling domain
location to be defined with spatiotemporal control. We can also use the laser to select
individual vesicles in a population to undergo mixing/demixing by locally heating the
membrane through the miscibility transition, demonstrating a further layer of control. This
technology has potential as a tool to shed light on domain biophysics, on their role in biology,
and in sculpting membrane assemblies with user-defined membrane patterning.
Funding
This work was supported by the EPSRC via grant EP/J017566/1 and by EPSRC Fellowship EP/N016998/1 awarded to Y.E.
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Communications ChemistryVolume
2Issue
1Citation
FRIDDIN, M.S. ... et al., 2019. Direct manipulation of liquid ordered lipid membrane domains using optical traps. Communications Chemistry, 2: 6.Publisher
© the Authors. Published by Springer NatureVersion
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/ by/4.0/Acceptance date
2018-11-16Publication date
2019-01-29Notes
This is an Open Access Article. It is published bySpringer under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/eISSN
2399-3669Publisher version
Language
- en