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Do media extracellular vesicles and extracellular vesicles bound to the extracellular matrix represent distinct types of vesicles?

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posted on 2024-01-05, 12:41 authored by Saida Mebarek, Rene Buchet, Slawomir Pikula, Agnieszka Strzelecka-Kiliszek, Leyre Brizuela, Giada Corti, Federica Collacchi, Genevieve Anghieri, Andrea Magrini, Pietro Ciancaglini, Jose Luis Millan, Owen DaviesOwen Davies, Massimo Bottini
Mineralization-competent cells, including hypertrophic chondrocytes, mature osteoblasts, and osteogenic-differentiated smooth muscle cells secrete media extracellular vesicles (media vesicles) and extracellular vesicles bound to the extracellular matrix (matrix vesicles). Media vesicles are purified directly from the extracellular medium. On the other hand, matrix vesicles are purified after discarding the extracellular medium and subjecting the cells embedded in the extracellular matrix or bone or cartilage tissues to an enzymatic treatment. Several pieces of experimental evidence indicated that matrix vesicles and media vesicles isolated from the same types of mineralizing cells have distinct lipid and protein composition as well as functions. These findings support the view that matrix vesicles and media vesicles released by mineralizing cells have different functions in mineralized tissues due to their location, which is anchored to the extracellular matrix versus free-floating.

Funding

Italian Ministry for Research [2022 Program for Research Projects of National Interest (PRIN), Grant # 20229RMZZW]

ERA-CVD/MICROEXPLORATION/ 4/2018 grant from the National Centre for Research and Development, Poland

Hubert Curien Partnership Programme POLONIUM # PPN/BFR/2020/1/00056 for years 2021/2022 co-financed by the Polish National Agency for Academic Exchange and the Ministry of Foreign Affairs and International Development of France

Nencki Institute of Experimental Biology, Polish Academy of Sciences

France-UK Hubert Curien Partnership Programme ALLIANCE

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, grants 2019/08568-2)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Finance Code 001) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 305426/2021-4)

RESTORE: engineeRing an Enhanced vesicle SysTem for coOrdinated fRacture rEpair

Engineering and Physical Sciences Research Council

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Deciphering molecular crosstalk in the musculoskeletal system: vesicle-mediated communication and its impact on bone health

Academy of Medical Sciences

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History

School

  • Sport, Exercise and Health Sciences

Published in

Biomolecules

Volume

14

Issue

1

Publisher

MDPI

Version

  • VoR (Version of Record)

Rights holder

© the authors

Publisher statement

This is an Open Access Article. It is published by MDPI under the Creative Commons Attribution 4.0 International Licence (CC BY). Full details of this licence are available at: https://creativecommons.org/licenses/by/4.0/

Acceptance date

2023-12-22

Publication date

2023-12-28

Copyright date

2023

DOI

eISSN

2218-273X

Publisher version

Language

  • en

Depositor

Dr Owen Davies. Deposit date: 4 January 2024

Article number

42

Usage metrics

    Loughborough Publications

    Categories

    Keywords

    Media extracellular vesiclesMatrix vesiclesCell-cell communicationBiomineralizationCollagenaseUltracentrifugation

    Licence

    CC BY 4.0

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