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Effect of reduced culture temperature on antioxidant defences of mesenchymal stem cells
journal contribution
posted on 2015-03-04, 12:05 authored by Alexandra StolzingAlexandra Stolzing, Andrew ScuttMesenchymal stem cells (MSC) promise to be valuable therapeutic tools but, due to their low numbers, require considerable in vitro expansion before use. This leads to in vitro aging, the accumulation of intracellular oxidative damage, and subsequently a decreased potential for proliferation and differentiation. Optimised culture conditions might help to reduce oxidative damage in MSC in vitro, and therefore, as reduced temperature is known to reduce oxidative stress in other somatic cells, we have investigated the effect of reduced temperature on rat MSC viability, differentiation, and oxidative damage. Temperature reduction did not affect MSC viability but increased differentiation and reduced apoptosis. Oxidative-damage-related indices were improved; reactive oxide species, nitric oxide, thiobarbituric acid reactive substances, carbonyl, and lipofuscin levels were reduced and glutathione peroxidase and superoxide dimutase levels increased. Levels of antiapoptotic heat shock proteins (HSP-27, -70, and -90) were raised and levels of the proapoptotic HSP-60 reduced. These data demonstrate that culturing MSC at reduced temperature decreases the accumulation of oxidative damage and therefore would probably improve long-term viability and successful engraftment of MSC used for tissue engineering or cell therapeutic purposes. © 2006.
Funding
BBSRC
History
School
- Mechanical, Electrical and Manufacturing Engineering
Published in
Free Radical Biology and MedicineVolume
41Issue
2Pages
326 - 338Citation
STOLZING, A. and SCUTT, A., 2006. Effect of reduced culture temperature on antioxidant defences of mesenchymal stem cells. Free Radical Biology and Medicine, 41 (2), pp. 326 - 338.Publisher
© Elsevier Inc.Version
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2006Notes
Closed accessISSN
0891-5849Publisher version
Other identifier
S0891584906002796Language
- en