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Effect of reduced culture temperature on antioxidant defences of mesenchymal stem cells

journal contribution
posted on 04.03.2015, 12:05 by Alexandra StolzingAlexandra Stolzing, Andrew Scutt
Mesenchymal stem cells (MSC) promise to be valuable therapeutic tools but, due to their low numbers, require considerable in vitro expansion before use. This leads to in vitro aging, the accumulation of intracellular oxidative damage, and subsequently a decreased potential for proliferation and differentiation. Optimised culture conditions might help to reduce oxidative damage in MSC in vitro, and therefore, as reduced temperature is known to reduce oxidative stress in other somatic cells, we have investigated the effect of reduced temperature on rat MSC viability, differentiation, and oxidative damage. Temperature reduction did not affect MSC viability but increased differentiation and reduced apoptosis. Oxidative-damage-related indices were improved; reactive oxide species, nitric oxide, thiobarbituric acid reactive substances, carbonyl, and lipofuscin levels were reduced and glutathione peroxidase and superoxide dimutase levels increased. Levels of antiapoptotic heat shock proteins (HSP-27, -70, and -90) were raised and levels of the proapoptotic HSP-60 reduced. These data demonstrate that culturing MSC at reduced temperature decreases the accumulation of oxidative damage and therefore would probably improve long-term viability and successful engraftment of MSC used for tissue engineering or cell therapeutic purposes. © 2006.

Funding

BBSRC

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Free Radical Biology and Medicine

Volume

41

Issue

2

Pages

326 - 338

Citation

STOLZING, A. and SCUTT, A., 2006. Effect of reduced culture temperature on antioxidant defences of mesenchymal stem cells. Free Radical Biology and Medicine, 41 (2), pp. 326 - 338.

Publisher

© Elsevier Inc.

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2006

Notes

Closed access

ISSN

0891-5849

Other identifier

S0891584906002796

Language

en