aging-v14i5-203931.pdf (1.59 MB)
Download fileEnhanced co-culture and enrichment of human natural killer cells for the selective clearance of senescent cells
journal contribution
posted on 2022-02-25, 10:23 authored by Kristie Kim, Tesfahun Dessale Admasu, Alexandra StolzingAlexandra Stolzing, Amit SharmaIn the context of aging and age-associated diseases, Natural Killer (NK) cells have been revealed as a key cell
type responsible for the immune clearance of senescent cells. Subsequently, NK cell-based therapies have
emerged as promising alternatives to drug-based therapeutic interventions for the prevention and treatment of
age-related disease and debility. Given the promise of NK cell-mediated immunotherapies as a safe and
effective treatment strategy, we outline an improved method by which primary NK cells can be efficiently
enriched from human peripheral blood across multiple donors (ages 20-42 years old), with a practical protocol
that reliably enhances both CD56dim and CD56bright NK cells by 15-fold and 3-fold, respectively. Importantly, we
show that our co-culture protocol can be used as an easily adaptable tool to assess highly efficient and selective
killing of senescent cells by primary NK cells enriched via our method using longer co-culture durations and a
low target to effector ratio, which may be more physiological than has been achieved in previous literature.
Funding
National Academy of Medicine (NAM) Healthy Longevity Catalyst Award # 2000011734
History
School
- Mechanical, Electrical and Manufacturing Engineering
Published in
AgingVolume
14Issue
5Pages
2131 - 2147Publisher
Impact JournalsVersion
- VoR (Version of Record)
Rights holder
© the AuthorsPublisher statement
This is an Open Access Article. It is published by Impact Journals under the Creative Commons Attribution 3.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/Acceptance date
2022-02-22Publication date
2022-03-04Copyright date
2022ISSN
1945-4589Publisher version
Language
- en