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Enhanced co-culture and enrichment of human natural killer cells for the selective clearance of senescent cells

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journal contribution
posted on 25.02.2022, 10:23 authored by Kristie Kim, Tesfahun Dessale Admasu, Alexandra StolzingAlexandra Stolzing, Amit Sharma
In the context of aging and age-associated diseases, Natural Killer (NK) cells have been revealed as a key cell type responsible for the immune clearance of senescent cells. Subsequently, NK cell-based therapies have emerged as promising alternatives to drug-based therapeutic interventions for the prevention and treatment of age-related disease and debility. Given the promise of NK cell-mediated immunotherapies as a safe and effective treatment strategy, we outline an improved method by which primary NK cells can be efficiently enriched from human peripheral blood across multiple donors (ages 20-42 years old), with a practical protocol that reliably enhances both CD56dim and CD56bright NK cells by 15-fold and 3-fold, respectively. Importantly, we show that our co-culture protocol can be used as an easily adaptable tool to assess highly efficient and selective killing of senescent cells by primary NK cells enriched via our method using longer co-culture durations and a low target to effector ratio, which may be more physiological than has been achieved in previous literature.

Funding

National Academy of Medicine (NAM) Healthy Longevity Catalyst Award # 2000011734

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Aging

Volume

14

Issue

5

Pages

2131 - 2147

Publisher

Impact Journals

Version

VoR (Version of Record)

Rights holder

© the Authors

Publisher statement

This is an Open Access Article. It is published by Impact Journals under the Creative Commons Attribution 3.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/

Acceptance date

22/02/2022

Publication date

2022-03-04

Copyright date

2022

ISSN

1945-4589

Language

en

Depositor

Prof Alexandra Stolzing. Deposit date: 25 February 2022