Taylor vortex and PolyILs protein crystallisation.pdf (5.24 MB)
Download fileEnhancement of protein crystallization with the application of Taylor vortex and Poly(ionic liquid)s
journal contribution
posted on 2022-05-06, 10:41 authored by Tiantian Tao, Zhenguo Gao, Chen Fang, Jiayin Zhang, Jun Xu, Huaiyu YangHuaiyu Yang, Junbo GongWith the increase demands for biopharmaceuticals, the development of efficient protein crystallization processes for manufacturing highly pure crystalline products has become critical for downstream biomanufacturing. This work focused on the combined effects of the uniform shear rate in a solution created by using the Taylor vortex and the macromolecular architecture of a solution developed using poly(ionic liquid)s during protein crystallization. The results were as follows: (1) the accelerated primary nucleation rate with a uniform shear force that was generated by the Taylor vortex; (2) the stability of the protein product prepared using the poly(ionic liquid)s was high in the crystallization solution; and (3) improved control of supersaturation of the polymer was achieved with the salting-out effect. The average crystal size in the control group was considerably lower than 1 μm with low quality (9.00 Å) and yield (56%) of crystals, but the average size of lysozyme crystals obtained using the Taylor vortex and poly(ionic liquid)s increased up to 5 μm with high quality (1.86 Å) of crystals and yield (86%). A higher diffraction resolution indicated a better ordered crystalline structure, demonstrating that the Taylor vortex and poly(ionic liquid)s are useful for improving the crystal quality.
Funding
National Natural Science Foundation of China, China (21938009, 22008173)
NSFC-RS (22111530115)
Royal Society International Exchanges (IEC\NSFC\201432)
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Chemical Engineering ScienceVolume
252Publisher
Elsevier BVVersion
- AM (Accepted Manuscript)
Rights holder
© ElsevierPublisher statement
This paper was accepted for publication in the journal Chemical Engineering Science and the definitive published version is available at https://doi.org/10.1016/j.ces.2022.117501Acceptance date
2022-02-05Publication date
2022-02-10Copyright date
2022ISSN
0009-2509eISSN
1873-4405Publisher version
Language
- en