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Enhancement of protein crystallization with the application of Taylor vortex and Poly(ionic liquid)s

journal contribution
posted on 06.05.2022, 10:41 authored by Tiantian Tao, Zhenguo Gao, Chen Fang, Jiayin Zhang, Jun Xu, Huaiyu YangHuaiyu Yang, Junbo Gong
With the increase demands for biopharmaceuticals, the development of efficient protein crystallization processes for manufacturing highly pure crystalline products has become critical for downstream biomanufacturing. This work focused on the combined effects of the uniform shear rate in a solution created by using the Taylor vortex and the macromolecular architecture of a solution developed using poly(ionic liquid)s during protein crystallization. The results were as follows: (1) the accelerated primary nucleation rate with a uniform shear force that was generated by the Taylor vortex; (2) the stability of the protein product prepared using the poly(ionic liquid)s was high in the crystallization solution; and (3) improved control of supersaturation of the polymer was achieved with the salting-out effect. The average crystal size in the control group was considerably lower than 1 μm with low quality (9.00 Å) and yield (56%) of crystals, but the average size of lysozyme crystals obtained using the Taylor vortex and poly(ionic liquid)s increased up to 5 μm with high quality (1.86 Å) of crystals and yield (86%). A higher diffraction resolution indicated a better ordered crystalline structure, demonstrating that the Taylor vortex and poly(ionic liquid)s are useful for improving the crystal quality.

Funding

Regenerative BioCrystallisation

Engineering and Physical Sciences Research Council

Find out more...

National Natural Science Foundation of China, China (21938009, 22008173)

NSFC-RS (22111530115)

Royal Society International Exchanges (IEC\NSFC\201432)

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Chemical Engineering Science

Volume

252

Publisher

Elsevier BV

Version

AM (Accepted Manuscript)

Rights holder

© Elsevier

Publisher statement

This paper was accepted for publication in the journal Chemical Engineering Science and the definitive published version is available at https://doi.org/10.1016/j.ces.2022.117501

Acceptance date

05/02/2022

Publication date

2022-02-10

Copyright date

2022

ISSN

0009-2509

eISSN

1873-4405

Language

en

Depositor

Dr Huaiyu Yang. Deposit date: 4 May 2022

Article number

117501