EuroTracker dyes_2015_.pdf (849.68 kB)
EuroTracker (R) dyes: design, synthesis, structure and photophysical properties of very bright europium complexes and their use in bioassays and cellular optical imaging
journal contribution
posted on 2015-09-15, 13:05 authored by Stephen ButlerStephen Butler, Martina Delbianco, Laurent Lamarque, Brian K. McMahon, Emily R. Neil, Robert Pal, David Parker, James W. Walton, Jurriaan M. ZwierThe development of the brightest luminescent europium(III) complexes is traced, including analysis of
the C3-symmetric core complex based on a functionalized triazacyclononane and identification of the
most suitable strongly absorbing chromophore. Strategies for the synthesis of the complexes, including
enantiopure analogues, are outlined and opportunities for applications in time-resolved microscopy and
spectral imaging emphasised. Practicable examples are introduced, including selective organelle staining
for cellular optical imaging at 65 nm resolution and the development of new bioassays using time resolved
FRET methods.
Funding
This work was supported by the ERC (SJB, ERN, RP, DP; FCC 266804), EC and EPSRC.
History
School
- Science
Department
- Chemistry
Published in
DALTON TRANSACTIONSVolume
44Issue
11Pages
4791 - 4803 (13)Citation
BUTLER, S.J. ... et al, 2015. EuroTracker (R) dyes: design, synthesis, structure and photophysical properties of very bright europium complexes and their use in bioassays and cellular optical imaging. Dalton Transactions, 44 (11), pp. 4791 - 4803.Publisher
© The Royal Society of ChemistryVersion
- NA (Not Applicable or Unknown)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2015Notes
This article was accepted for publication in the journal, Dalton Transactions [© Royal Society of Chemistry]. The definitive version is available at: http://dx.doi.org/10.1039/c4dt02785jISSN
1477-9226Publisher version
Language
- en