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Exercise duration-matched interval and continuous sprint cycling induce similar increases in AMPK phosphorylation, PGC-1α and VEGF mRNA expression in trained individuals

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posted on 05.07.2016, 15:51 by Conor W. Taylor, Stephen A. Ingham, Julie E.A. Hunt, Neil MartinNeil Martin, Jamie S.M. Pringle, Richard FergusonRichard Ferguson
Purpose: The effects of low-volume interval and continuous ‘all-out’ cycling, matched for total exercise duration, on mitochondrial and angiogenic cell signalling was investigated in trained individuals. Methods: In a repeated measures design, 8 trained males ((Formula presented.), 57 ± 7 ml kg−1 min−1) performed two cycling exercise protocols; interval (INT, 4 × 30 s maximal sprints interspersed by 4 min passive recovery) or continuous (CON, 2 min continuous maximal sprint). Muscle biopsies were obtained before, immediately after and 3 h post-exercise. Results: Total work was 53 % greater (P = 0.01) in INT compared to CON (71.2 ± 7.3 vs. 46.3 ± 2.7 kJ, respectively). Phosphorylation of AMPKThr172 increased by a similar magnitude (P = 0.347) immediately post INT and CON (1.6 ± 0.2 and 1.3 ± 0.3 fold, respectively; P = 0.011), before returning to resting values at 3 h post-exercise. mRNA expression of PGC-1α (7.1 ± 2.1 vs. 5.5 ± 1.8 fold; P = 0.007), VEGF (3.5 ± 1.2 vs. 4.3 ± 1.8 fold; P = 0.02) and HIF-1α (2.0 ± 0.5 vs. 1.5 ± 0.3 fold; P = 0.04) increased at 3 h post-exercise in response to INT and CON, respectively; the magnitude of which were not different between protocols. Conclusions: Despite differences in total work done, low-volume INT and CON ‘all-out’ cycling, matched for exercise duration, provides a similar stimulus for the induction of mitochondrial and angiogenic cell signalling pathways in trained skeletal muscle.

History

School

  • Sport, Exercise and Health Sciences

Published in

European Journal of Applied Physiology

Pages

1 - 10

Citation

TAYLOR, C.W. ... et al, 2016. Exercise duration-matched interval and continuous sprint cycling induce similar increases in AMPK phosphorylation, PGC-1α and VEGF mRNA expression in trained individuals. European Journal of Applied Physiology, 116 (8), pp. 1445-1454.

Publisher

Springer / © The Authors

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/ by/4.0/

Publication date

2016

Notes

This is an open access article published by Springer and distributed under the terms of the Creative Commons Attribution Licence (CC BY 4.0), https://creativecommons.org/licenses/by/4.0/

ISSN

1439-6319

Language

en