Hanga_et_al-2017-Journal_of_Chemical_Technology_and_Biotechnology.pdf (10.35 MB)
Download fileExpansion of bone marrow‐derived human mesenchymal stem/stromal cells (hMSCs) using a two‐phase liquid/liquid system
journal contribution
posted on 2017-04-25, 10:44 authored by Mariana P. Hanga, Halina Murasiewicz, Andrzej W. Pacek, Alvin W. Nienow, Karen CoopmanKaren Coopman, Christopher J. HewittBACKGROUND: Humanmesenchymal stem/stromal cells (hMSCs) are at the forefront of regenerativemedicine applications due to their relatively easy isolation and availability in adults, potential to differentiate and to secrete a range of trophic factors that could determine specialised tissue regeneration. To date, hMSCs have been successfully cultured in vitro on substrates such as polystyrene dishes (TCPS) or microcarriers. However, hMSC sub-cultivation and harvest typically employs proteolytic enzymes that act by cleaving important cell membrane proteins resulting in long-term cell damage. In a processwhere the cells themselves are the product, a non-enzymatic and non-damaging harvesting approach is desirable. RESULTS: An alternative system for hMSC expansion and subsequent non-enzymatic harvest was investigated here. A liquid/liquid two-phase system was proposed, comprising a selected perfluorocarbon (FC40) and growth medium (DMEM). The cells exhibited similar cell morphologies compared with TCPS. Moreover, they retained their identity and differentiation potential post-expansion and post-harvest. Further, no significant difference was found when culturing hMSCs in the culture systems prepared with either fresh or recycled FC40 perfluorocarbon.
CONCLUSIONS: These findings make the FC40/DMEM system an attractive alternative for traditional cell culture substrates due
to their ease of cell recovery and recyclability, the latter impacting on overall process costs.
Funding
This work was possible thanks to the funding received from BBSRC and the Bioprocessing Research Industry Club (BRIC BB/K011066/1 and BB/K01099/1).
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Journal of Chemical Technology & BiotechnologyVolume
92Issue
7Pages
1577 - 1589Citation
HANGA, M.P. ...et al., 2017. Expansion of bone marrow‐derived human mesenchymal stem/stromal cells (hMSCs) using a two‐phase liquid/liquid system. Journal of Chemical Technology & Biotechnology, 92 (7), pp.1577–1589Publisher
John Wiley & Sons Ltd on behalf of Society of Chemical Industry (© The Authors)Version
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/Acceptance date
2017-03-09Publication date
2017-04-24Notes
This is an Open Access Article. It is published by Wiley under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/ISSN
0268-2575eISSN
1097-4660Publisher version
Language
- en