Expansion of bone marrow‐derived human mesenchymal stem/stromal cells (hMSCs) using a two‐phase liquid/liquid system
journal contributionposted on 2017-04-25, 10:44 authored by Mariana P. Hanga, Halina Murasiewicz, Andrzej W. Pacek, Alvin W. Nienow, Karen CoopmanKaren Coopman, Christopher J. Hewitt
BACKGROUND: Humanmesenchymal stem/stromal cells (hMSCs) are at the forefront of regenerativemedicine applications due to their relatively easy isolation and availability in adults, potential to differentiate and to secrete a range of trophic factors that could determine specialised tissue regeneration. To date, hMSCs have been successfully cultured in vitro on substrates such as polystyrene dishes (TCPS) or microcarriers. However, hMSC sub-cultivation and harvest typically employs proteolytic enzymes that act by cleaving important cell membrane proteins resulting in long-term cell damage. In a processwhere the cells themselves are the product, a non-enzymatic and non-damaging harvesting approach is desirable. RESULTS: An alternative system for hMSC expansion and subsequent non-enzymatic harvest was investigated here. A liquid/liquid two-phase system was proposed, comprising a selected perfluorocarbon (FC40) and growth medium (DMEM). The cells exhibited similar cell morphologies compared with TCPS. Moreover, they retained their identity and differentiation potential post-expansion and post-harvest. Further, no significant difference was found when culturing hMSCs in the culture systems prepared with either fresh or recycled FC40 perfluorocarbon. CONCLUSIONS: These findings make the FC40/DMEM system an attractive alternative for traditional cell culture substrates due to their ease of cell recovery and recyclability, the latter impacting on overall process costs.
This work was possible thanks to the funding received from BBSRC and the Bioprocessing Research Industry Club (BRIC BB/K011066/1 and BB/K01099/1).
- Aeronautical, Automotive, Chemical and Materials Engineering
- Chemical Engineering
Published inJournal of Chemical Technology & Biotechnology
Pages1577 - 1589
CitationHANGA, M.P. ...et al., 2017. Expansion of bone marrow‐derived human mesenchymal stem/stromal cells (hMSCs) using a two‐phase liquid/liquid system. Journal of Chemical Technology & Biotechnology, 92 (7), pp.1577–1589
PublisherJohn Wiley & Sons Ltd on behalf of Society of Chemical Industry (© The Authors)
- VoR (Version of Record)
Publisher statementThis work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/
NotesThis is an Open Access Article. It is published by Wiley under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/