Hewitt-expansion of human mesenchymal.pdf (438.05 kB)
Download fileExpansion of human mesenchymal stem cells on microcarriers
journal contribution
posted on 2011-12-06, 14:15 authored by Christopher Hewitt, Kenneth Lee, Alvin W. Nienow, Rob ThomasRob Thomas, M. Smith, Colin R. ThomasThe effects on human mesenchymal stem cell growth of choosing either of two spinner flask impeller geometries, two microcarrier concentrations and two cell concentrations (seeding densities) were investigated. Cytodex 3 microcarriers were not damaged when held at the minimum speed, NJS, for their suspension, using either impeller, nor was there any observable damage to the cells. The maximum cell density was achieved after 8–10 days of culture with up to a 20-fold expansion in terms of cells per microcarrier. An increase in microcarrier concentration or seeding density generally had a deleterious or neutral effect, as previously observed for human fibroblast cultures. The choice of impeller was significant, as was incorporation of a 1 day delay before agitation to allow initial attachment of cells. The best conditions for cell expansion on the microcarriers in the flasks were 3,000 microcarriers ml−1 (ca. 1 g dry weight l−1), a seeding density of 5 cells per microcarrier with a 1 day delay before agitation began at NJS (30 rpm), using a horizontally suspended flea impeller with an added vertical paddle. These findings were interpreted using Kolmogorov’s theory of isotropic turbulence.
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Biotechnology LettersVolume
33Issue
11Pages
2325 - 2335Citation
HEWITT, C.J. ... et al, 2011. Expansion of human mesenchymal stem cells on microcarriers. Biotechnology Letters, 33 (11), pp. 2325-2335.Publisher
© Springer VerlagVersion
- AM (Accepted Manuscript)
Publication date
2011Notes
This article was published in the journal, Biotechnology Letters [© Springer Verlag]. The definitive version is available at: http://www.springerlink.com/content/57t4lj6553956p07/ISSN
0141-5492Publisher version
Language
- en