Accepted version of manuscript.pdf (1.61 MB)
Exploration of matrix effects in laser ablation inductively coupled plasma mass spectrometry imaging of cisplatin treated tumors
journal contribution
posted on 2020-06-25, 09:13 authored by Calum Greenhalgh, Ellie Karekla, Gareth J Miles, Ian R Powley, Catia Costa, Janella de Jesus, Melanie Jane Bailey, Catrin Pritchard, Marion MacFarlane, J Howard Pringle, Amy ManaghAmy ManaghThe use of a low aerosol dispersion ablation chamber within a LA-ICP-MS set up allows for high-resolution, high-speed imaging of the distribution of elements within a sample. Here we show how this enhanced capability creates new analytical problems and solutions. We report the distribution of platinum at the cellular level in non-small cell lung cancer (NSCLC) explant models after treatment with clinically relevant doses of cisplatin. This revealed for the first time a correlation between the platinum signal and the presence of carbon deposits within lung tissue. We show how complementary ion beam analysis techniques, particle induced X-ray emission (PIXE) and elastic backscattering spectrometry (EBS) can be used to explore potential matrix effects in LA-ICP-MS data. For these samples, it was confirmed that the enhancement was unlikely to have resulted from a matrix effect alone. Thus, the presence of carbon deposits within tissue has potential implications for the effective distribution of the cisplatin drug.
Funding
C. Greenhalgh would like to thank Loughborough University for the provision of a funded PhD studentship. Preparation of explants was supported by a Medical Research Council (MRC) Doctoral Training Grant to E. Karekla, the MRC Toxicology Unit (MC A/600), and the Leicester Experimental Cancer Medicine Centre (C325/A15575 Cancer Research UK/UK Department of Health). C. Pritchard was supported by a Royal Society- Wolfson merit award. Access to the UK National Ion beam Centre was provided through an EPSRC pump primer grant (project 579)
History
School
- Science
Department
- Chemistry
Published in
Analytical ChemistryVolume
92Issue
14Pages
9847 - 9855Publisher
American Chemical SocietyVersion
- AM (Accepted Manuscript)
Rights holder
© American Chemical SocietyPublisher statement
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.analchem.0c01347.Acceptance date
2020-06-17Publication date
2020-06-17Copyright date
2020ISSN
0003-2700eISSN
1520-6882Publisher version
Language
- en
Depositor
Dr Amy Managh. Deposit date: 24 June 2020Usage metrics
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