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Exploring biochemical pathways for mono-ethylene glycol (MEG) synthesis from synthesis gas
journal contribution
posted on 2017-11-07, 11:27 authored by Ahsan IslamAhsan Islam, Noushin Hadadi, Meric Ataman, Vassily Hatzimanikatis, Gregory StephanopoulosMono-ethylene glycol (MEG) is an important petrochemical with widespread use in numerous consumer products. The current industrial MEG-production process relies on non-renewable fossil fuel-based feedstocks, such as petroleum, natural gas, and naphtha; hence, it is useful to explore alternative routes of MEG-synthesis from gases as they might provide a greener and more sustainable alternative to the current production methods. Technologies of synthetic biology and metabolic engineering of microorganisms can be deployed for the expression of new biochemical pathways for MEG-synthesis from gases, provided that such promising alternative routes are first identified. We used the BNICE.ch algorithm to develop novel and previously unknown biological pathways to MEG from synthesis gas by leveraging the Wood-Ljungdahl pathway of carbon fixation of acetogenic bacteria. We developed a set of useful pathway pruning and analysis criteria to systematically assess thousands of pathways generated by BNICE.ch. Published genome-scale models of Moorella thermoacetica and Clostridium ljungdahlii were used to perform the pathway yield calculations and in-depth analyses of seven (7) newly developed biological MEG-producing pathways from gases, including CO2, CO, and H2. These analyses helped identify not only better candidate pathways, but also superior chassis organisms that can be used for metabolic engineering of the candidate pathways. The pathway generation, pruning, and detailed analysis procedures described in this study can also be used to develop biochemical pathways for other commodity chemicals from gaseous substrates.
Funding
MAI and GS would like to thank the SABIC Americas, Inc. for funding the project. VH, NH and MA acknowledge the Swiss National Science Foundation (SNF) and SystemsX.ch, the Swiss Initiative in Systems Biology for funding the project.
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Metabolic EngineeringVolume
41Pages
173 - 181Citation
ISLAM, M. ... et al., 2017. Exploring biochemical pathways for mono-ethylene glycol (MEG) synthesis from synthesis gas. Metabolic Engineering, 41, pp.173-181.Publisher
Elsevier (© International Metabolic Engineering Society)Version
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 2.5 Generic (CC BY-NC-ND 2.5) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by-nc-nd/2.5/Acceptance date
2017-04-16Publication date
2017-04-19ISSN
1096-7176Publisher version
Language
- en