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Fusing artificial cell compartments and lipid domains using optical traps: a tool to modulate membrane composition and phase behaviour

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journal contribution
posted on 05.03.2021, 17:03 by Adithya Vivek, Guido BolognesiGuido Bolognesi, Yuval Elani
New technologies for manipulating biomembranes have vast potential to aid the understanding of biological phenomena, and as tools to sculpt novel artificial cell architectures for synthetic biology. The manipulation and fusion of vesicles using optical traps is amongst the most promising due to the level of spatiotemporal control it affords. Herein, we conduct a suite of feasibility studies to show the potential of optical trapping technologies to (i) modulate the lipid composition of a vesicle by delivering new membrane material through fusion events and (ii) manipulate and controllably fuse coexisting membrane domains for the first time. We also outline some noteworthy morphologies and transitions that the vesicle undergoes during fusion, which gives us insight into the mechanisms at play. These results will guide future exploitation of laser-assisted membrane manipulation methods and feed into a technology roadmap for this emerging technology.

Funding

Engineering Living/Synthetic Hybrid Assemblies (LSHAs) as Functional Units for Synthetic Biology

Engineering and Physical Sciences Research Council

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An engineering rulebook for interfacing living and non-living cells

UK Resarch and Innovation

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History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Micromachines

Volume

11

Issue

4

Publisher

MDPI AG

Version

VoR (Version of Record)

Rights holder

© The authors

Publisher statement

This is an Open Access Article. It is published by MDPI under the Creative Commons Attribution 4.0 International Licence (CC BY 4.0). Full details of this licence are available at: https://creativecommons.org/licenses/by/4.0/

Acceptance date

28/03/2020

Publication date

2020-04-07

Copyright date

2020

ISSN

2072-666X

eISSN

2072-666X

Language

en

Depositor

Dr Guido Bolognesi. Deposit date: 5 March 2021

Article number

388