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Glucose-induced replicative senescence in mesenchymal stem cells

journal contribution
posted on 05.03.2015, 13:42 by Alexandra StolzingAlexandra Stolzing, Natalie Coleman, Andrew Scutt
Mesenchymal stem cells (MSCs) show great promise for use in a variety of cell-based therapies. Because isolated primary mesenchymal stem cells are low in numbers, in vitro expansion is necessary. However, the expansion potential is limited and in vitro aging leads to loss of multipotency and replicative senescence. Stress induced by culture conditions is likely to be a major cause of replicative senescence and reduced multipotency of MSC and optimization of culture conditions might be able to reduce this. Caloric restriction (CR) is the only established method to delay aging and extend lifespan. In vitro caloric restriction experiments are rare, but have demonstrated beneficial effects. Therefore, we investigated the effect of culture medium glucose concentration on the proliferative and differentiation potential of mesenchymal stem cells. Reduction in glucose concentrations led to decreased apoptosis and an increased rate of MSC proliferation and increased the number and size of fibroblastic colonies in the colony-forming unit assay. © Mary Ann Liebert, Inc.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Rejuvenation Research

Volume

9

Issue

1

Pages

31 - 35

Citation

STOLZING, A., COLEMAN, N. and SCUTT, A., 2006. Glucose-induced replicative senescence in mesenchymal stem cells. Rejuvenation Research, 9 (1), pp. 31 - 35.

Publisher

© Mary Ann Liebert, Inc.

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2006

Notes

This article is closed access.

ISSN

1549-1684

Language

en

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