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Gold nanoparticle interactions in human blood: a model evaluation

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journal contribution
posted on 06.12.2018, 11:39 by Niloofar Ajdari, Cian Vyas, Stephanie L. Bogan, Bashir A. Lwaleed, Brian CousinsBrian Cousins
In this study, we investigated gold nanoparticle (AuNP) interactions in blood using thromboelastography as a rapid screening tool to monitor their influence on blood coagulation. 1.2 nM colloidal AuNPs ranging from 12 to 85 nm have no effect in the blood, however, 5 nM AuNPs demonstrate pro-thrombogenic concentration dependent effects with a reduction in clot formation. Size effects exhibit a non-linear trend with 45 and 85 nm particles resulting in a faster pro-thrombotic response. Clot strength decreased with AuNP size with the greatest reduction with 28 nm particles. We assessed AuNP interactions in the blood focusing on their biological activity. AuNP-RGD possessed procoagulant activities, while PEG-thiol, human fibrinogen and clopidogrel prevented blood clot formation and influenced platelet activity, and were more efficient when bound to nanocarriers than unbound ligands. Such tests could fill the knowledge gaps in thrombogenicity of NPs between in vitro test methods and predict in vivo haemocompatibility.

Funding

The authors would like to acknowledge the financial support of the EPSRC CASE Award (EP/ L504889/1), and Healthcare Impact Partnership (EP/L024713/1).

History

School

  • Science

Department

  • Chemistry

Published in

Nanomedicine: Nanotechnology, Biology and Medicine

Volume

13

Issue

4

Pages

1531 - 1542

Citation

AJDARI, N. ... et al., 2017. Gold nanoparticle interactions in human blood: a model evaluation. Nanomedicine: Nanotechnology, Biology and Medicine, 13(4), pp. 1531-1542.

Publisher

© the Authors. Published by Elsevier

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/ by/4.0/

Acceptance date

31/01/2017

Publication date

2017-02-24

Notes

This is an Open Access Article. It is published by Elsevier under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/

ISSN

1549-9634

Language

en