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High magnesium corrosion rate has an effect on osteoclast and mesenchymal stem cell role during bone remodelling

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posted on 23.07.2018, 15:25 authored by Diana Maradze, David Musson, Yufeng Zheng, Jillian Cornish, Mark LewisMark Lewis, Yang LiuYang Liu
The aim of this study was to gain an understanding on the collective cellular effects of magnesium (Mg) corrosion products on the behaviour of cells responsible for bone formation and remodelling. The response of mesenchymal stem cells (MSCs) and osteoclast cells to both soluble (Mg ions) and insoluble (granule) corrosion products were recapitulated in vitro by controlling the concentration of the corrosion products. Clearance of corrosion granules by MSCs was also inspected by TEM analysis at sub-cellular level. The effect of Mg corrosion products varied depending on the state of differentiation of cells, concentration and length of exposure. The presence of the corrosion products significantly altered the cells' metabolic and proliferative activities, which further affected cell fusion/differentiation. While cells tolerated higher than physiological range of Mg concentration (16 mM), concentrations below 10 mM were beneficial for cell growth. Furthermore, MSCs were shown to contribute to the clearance of intercellular corrosion granules, whilst high concentrations of corrosion products negatively impacted on osteoclast progenitor cell number and mature osteoclast cell function.

Funding

DM’s PhD studentship was supported by The EPSRC (EP/F500491/1) Centre of Doctor Training in Regenerative Medicine and the exchange activity with University of Auckland was funded by SkelGen under Marie Sklodowska-Curie Research and Innovation Staff Exchange programme (FP7-PEOPLE-2012-IRSES, 318553).

History

School

  • Sport, Exercise and Health Sciences

Published in

Scientific Reports

Volume

8

Issue

1

Citation

MARADZE, D. ... et al, 2018. High magnesium corrosion rate has an effect on osteoclast and mesenchymal stem cell role during bone remodelling. Scientific Reports, 8 (1), 10003.

Publisher

Springer Nature © The Author(s)

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/ by/4.0/

Acceptance date

19/06/2018

Publication date

2018-07-03

Copyright date

2018

Notes

This is an Open Access Article. It is published by Springer Nature under the Creative Commons Attribution 4.0 International Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/

eISSN

2045-2322

Language

en

Article number

10003