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Highly manufacturable graphene oxide biosensor for sensitive Interleukin-6 detection

journal contribution
posted on 17.09.2015, 13:09 by Jingfeng Huang, Hu Chen, Wenbin Niu, Derrick W.H. Fam, Alagappan Palaniappan, Melanie Larisika, Steve Faulkner, Christoph Nowak, Myra A. Nimmo, Bo Liedberg, Alfred I.Y. Tok
Graphene Oxide (GO) is analogous to graphene with oxygen moieties. It offers several advantages over graphene, such as a tunable band-gap, facile synthesis and no use of metal catalysts. Due to the monolayer configuration of GO, all of its carbon atoms are readily exposed to the atmosphere and are sensitive to surface perturbations, thus making GO very suitable for liquid-gated field effect transistor (FET) type sensing applications. However, there are two main limitations preventing GO usage in practical FET sensors. It displays (1) variable coverage between fabricated chips and (2) high electrical resistance. In this paper, we overcome these two limitations by using a facile atmospheric-pressure ethanol Chemical Vapor Deposition treatment on top of pre-coated GO (ECVDGO) which decreases the electrical resistivity from 1.99 × 106 Ω square−1 to 4.68 × 103 Ω square−1, and resistivity variation from 1.60 × 106 to 7.72 × 102 Ω square−1; whilst enlarging the surface GO coverage up to 100%. We then demonstrate the ability of the post-treated ECVDGO liquid-gated FET transducer to detect Interleukin-6 which is a multi-functional cytokine involved in regulating the immune function and the acute phase response. The sensing window of the fabricated biosensor to Interleukin-6 is within the physiologically-relevant range, from 4.7 to 300 pg ml−1. The LOD of the sensor based on 3σ is 2.9 pA or 1.53 pg ml−1. This study demonstrates the emerging potential of GO with high manufacturability in liquid-gated FET biosensors for sensitive and label-free detection of bio-molecules.

Funding

The author is grateful for the research funding from the Institute for Sports Research (ISR) of Nanyang Technological University (NTU). The research was also partly supported by the National Institute for Health Research (NIHR) Diet, Lifestyle & Physical Activity Biomedical Research Unit based at University Hospitals of Leicester and Loughborough University.

History

School

  • Sport, Exercise and Health Sciences

Published in

RSC ADVANCES

Volume

5

Issue

49

Pages

39245 - 39251 (7)

Citation

HUANG, J. ... et al, 2015. Highly manufacturable graphene oxide biosensor for sensitive Interleukin-6 detection. RSC Advances, 5 (49), pp. 39245 - 39251.

Publisher

© The Royal Society of Chemistry

Version

VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2015

Notes

This article is closed access.

ISSN

2046-2069

Language

en

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