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In situ analysis of a silver nanoparticle-precipitating Shewanella biofilm by surface enhanced confocal Raman microscopy

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journal contribution
posted on 02.08.2019, 10:59 by Gal Schkolnik, Matthias Schmidt, Marco MazzaMarco Mazza, Falk Harnisch, Niculina Musat
Shewanella oneidensis MR-1 is an electroactive bacterium, capable of reducing extracellular insoluble electron acceptors, making it important for both nutrient cycling in nature and microbial electrochemical technologies, such as microbial fuel cells and microbial electrosynthesis. When allowed to anaerobically colonize an Ag/AgCl solid interface, S. oneidensis has precipitated silver nanoparticles (AgNp), thus providing the means for a surface enhanced confocal Raman microscopy (SECRaM) investigation of its biofilm. The result is the in-situ chemical mapping of the biofilm as it developed over time, where the distribution of cytochromes, reduced and oxidized flavins, polysaccharides and phosphate in the undisturbed biofilm is monitored. Utilizing AgNp bio-produced by the bacteria colonizing the Ag/ AgCl interface, we could perform SECRaM while avoiding the use of a patterned or roughened support or the introduction of noble metal salts and reducing agents. This new method will allow a spatially and temporally resolved chemical investigation not only of Shewanella biofilms at an insoluble electron acceptor, but also of other noble metal nanoparticle-precipitating bacteria in laboratory cultures or in complex microbial communities in their natural habitats.

Funding

Europäischer Fonds für regionale Entwicklung (EFRE) Sachsen grant number SAB 100112100 (http://www.eu-info.de/ foerderprogramme/strukturfonds/EFRE/)

Initiative and Networking Fund of the Helmholtz Association, funding number PD – 165 (http://www.helmholtz.de/ en/about_us/initiating_and_networking/)

Max Planck Society (http://www.mpg. de/en)

Helmholtz Centre for Environmental Research (http://www.ufz.de/)

Deutsche Forschungsgemeinschaft (SFB 937, project A20)

History

Department

  • Mathematical Sciences

Published in

PLoS ONE

Volume

10

Issue

12

Pages

e0145871 - e0145871

Publisher

Public Library of Science (PLoS)

Version

VoR (Version of Record)

Rights holder

© Schkolnik et al.

Publisher statement

This is an Open Access Article. It is published by PLoS under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/

Acceptance date

09/12/2015

Publication date

2015-12-28

Copyright date

2015

ISSN

1932-6203

eISSN

1932-6203

Language

en

Editor(s)

A. Al-Ahmad