In situ modification of chromatography adsorbents using cold atmospheric pressure plasmas
journal contributionposted on 2015-07-02, 13:55 authored by P. Olszewski, Thomas C. Willett, Eirini Theodosiou, Owen R.T. Thomas, James L. Walsh
Efficient manufacturing of increasingly sophisticated biopharmaceuticals requires the development of new breeds of chromatographic materials featuring two or more layers, with each layer affording different functions. This letter reports the in situ modification of a commercial beaded anion exchange adsorbent using atmospheric pressure plasma generated within gas bubbles. The results show that exposure to He-O2 plasma in this way yields significant reductions in the surface binding of plasmid DNA to the adsorbent exterior, with minimal loss of core protein binding capacity; thus, a bi-layered chromatography material exhibiting both size excluding and anion exchange functionalities within the same bead is produced.
This work is funded by the Biotechnology and Biological Sciences Research Council/Bioprocessing Research Industry Club (BRIC) programme (Grant No. BB/F004982/1), and the Engineering and Physical Sciences Research Council (Grant No. EP/J005894/1).
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