1.4807391.pdf (1.69 MB)
In situ modification of chromatography adsorbents using cold atmospheric pressure plasmas
journal contributionposted on 2015-07-02, 13:55 authored by P. Olszewski, Thomas C. Willett, Eirini Theodosiou, Owen R.T. Thomas, James L. Walsh
Efficient manufacturing of increasingly sophisticated biopharmaceuticals requires the development of new breeds of chromatographic materials featuring two or more layers, with each layer affording different functions. This letter reports the in situ modification of a commercial beaded anion exchange adsorbent using atmospheric pressure plasma generated within gas bubbles. The results show that exposure to He-O2 plasma in this way yields significant reductions in the surface binding of plasmid DNA to the adsorbent exterior, with minimal loss of core protein binding capacity; thus, a bi-layered chromatography material exhibiting both size excluding and anion exchange functionalities within the same bead is produced.
This work is funded by the Biotechnology and Biological Sciences Research Council/Bioprocessing Research Industry Club (BRIC) programme (Grant No. BB/F004982/1), and the Engineering and Physical Sciences Research Council (Grant No. EP/J005894/1).
- Aeronautical, Automotive, Chemical and Materials Engineering
- Chemical Engineering
Published inAPPLIED PHYSICS LETTERS
Pages? - ? (5)
CitationOLSZEWSKI, P. ... et al, 2013. In situ modification of chromatography adsorbents using cold atmospheric pressure plasmas. Applied Physics Letters, 102 (20), 204104.
Publisher© American Institute of Physics
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NotesCopyright 2013 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the authors and the American Institute of Physics. This article appeared in the journal, Applied Physics Letters, and may be found at: http://dx.doi.org/10.1063/1.4807391