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In situ modification of chromatography adsorbents using cold atmospheric pressure plasmas

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journal contribution
posted on 02.07.2015, 13:55 by P. Olszewski, Thomas C. Willett, Eirini Theodosiou, Owen R.T. Thomas, James L. Walsh
Efficient manufacturing of increasingly sophisticated biopharmaceuticals requires the development of new breeds of chromatographic materials featuring two or more layers, with each layer affording different functions. This letter reports the in situ modification of a commercial beaded anion exchange adsorbent using atmospheric pressure plasma generated within gas bubbles. The results show that exposure to He-O2 plasma in this way yields significant reductions in the surface binding of plasmid DNA to the adsorbent exterior, with minimal loss of core protein binding capacity; thus, a bi-layered chromatography material exhibiting both size excluding and anion exchange functionalities within the same bead is produced.

Funding

This work is funded by the Biotechnology and Biological Sciences Research Council/Bioprocessing Research Industry Club (BRIC) programme (Grant No. BB/F004982/1), and the Engineering and Physical Sciences Research Council (Grant No. EP/J005894/1).

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

APPLIED PHYSICS LETTERS

Volume

102

Issue

20

Pages

? - ? (5)

Citation

OLSZEWSKI, P. ... et al, 2013. In situ modification of chromatography adsorbents using cold atmospheric pressure plasmas. Applied Physics Letters, 102 (20), 204104.

Publisher

© American Institute of Physics

Version

VoR (Version of Record)

Publication date

2013

Notes

Copyright 2013 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the authors and the American Institute of Physics. This article appeared in the journal, Applied Physics Letters, and may be found at: http://dx.doi.org/10.1063/1.4807391

ISSN

0003-6951

Language

en

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Keywords

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