05062019_Peptide post review nohighlighted sections (1).pdf (1.57 MB)
Incorporating peptide aptamers into resistive pulse sensing
journal contribution
posted on 2020-02-13, 11:21 authored by Rhushabh Maugi, Zarina Salkenova, Mark PlattMark PlattThe use of nanocarriers within resistive pulse sensing, RPS, aids the detection and quantification of analytes. In the absence of convection, the signal strength and frequency can dependent upon the electrophoretic mobility of the nanocarrier/analyte. Here, we have developed a simple strategy to incorporate peptide aptamers onto RPS assays with enhanced electrophoretic signals. Using a hybrid DNA–Peptide nanocarrier, an existing peptide was incorporated into a rapid assay without having to engineer or modify the peptide sequence. The surface of a nanocarrier is coated with a mixture of peptide aptamers and a non‐binding DNA. The binding of the target to the peptide creates an “analyte corona” which shields the phosphate groups of the underlying DNA. This results in a change in electrophoretic mobility of the nanocarrier. The signal is concentration‐dependent and is illustrated using a peptide to a key biomarker of infection, C‐reactive protein, CRP. As a comparison, we also show the binding of the CRP to a DNA aptamer. This universal approach can be easily adapted to other peptides without the peptide itself to undergo any chemical modifications opening new opportunities and applications in RPS strategies.
History
School
- Science
Department
- Chemistry
Published in
Medical Devices & SensorsVolume
3Issue
1Publisher
WileyVersion
- AM (Accepted Manuscript)
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© Wiley Periodicals, Inc.Publisher statement
This is the peer reviewed version of the following article: MAUGI R., SALKENOVA Z. and PLATT M., 2020. Incorporating peptide aptamers into resistive pulse sensing. Medical Devices & Sensors, 3 (1), e10059, which has been published in final form at https://doi.org/10.1002/mds3.10059. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.Publication date
2020-01-18Copyright date
2020eISSN
2573-802XPublisher version
Language
- en
Depositor
Dr Mark Platt. Deposit date: 12 February 2020Article number
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