ac-2016-043156 Arthur et al_accepted manuscript and SI.pdf (925.89 kB)
Download fileIncreasing peak capacity in nontargeted omics applications by combining full scan field asymmetric waveform ion mobility spectrometry with liquid chromatography–mass spectrometry
journal contribution
posted on 2017-04-26, 08:52 authored by Kayleigh L. Arthur, Matthew TurnerMatthew Turner, Jim ReynoldsJim Reynolds, Colin CreaserFull scan field asymmetric waveform ion mobility spectrometry (FAIMS) combined with liquid chromatography and mass spectrometry (LC-FAIMS-MS) is shown to enhance peak capacity for omics applications. A miniaturized FAIMS device capable of rapid compensation field scanning has been incorporated into an ultrahigh performance liquid chromatography (UHPLC)
and time-of-flight mass spectrometry analysis, allowing the acquisition of full scan FAIMS and MS nested data sets within the time scale of a UHPLC peak. Proof of principle for the potential of scanning LC-FAIMS-MS in omics applications is demonstrated for the nontargeted profiling of human urine using a HILIC column. The high level of orthogonality between FAIMS and MS provides additional unique compound identifiers with detection of features based on retention time, FAIMS dispersion field and compensation field (DF and CF), and mass-to-charge (m/z). Extracted FAIMS full scan data can be matched to standards to aid the identification of unknown analytes. The peak capacity for features detected in human urine using LC-FAIMS-MS was increased approximately threefold compared to LC-MS alone due to a combination of the reduction of chemical noise and separation of coeluting isobaric species across the entire analytical space. The use of FAIMS-selected in source collision induced dissociation (FISCID) yields fragmentation of ions, which reduces sample complexity associated with overlapping fragmentation patterns and provides structural information on the selected precursor ions.
Funding
The authors thank Owlstone Limited and Loughborough University for financial support.
History
School
- Science
Department
- Chemistry
Published in
Analytical ChemistryVolume
89Issue
6Pages
3452 - 3459Citation
ARTHUR, K.L. ... et al, 2017. Increasing peak capacity in nontargeted omics applications by combining full scan field asymmetric waveform ion mobility spectrometry with liquid chromatography–mass spectrometry. Analytical Chemistry, 89 (6), pp. 3452-3459.Publisher
© American Chemical SocietyVersion
- AM (Accepted Manuscript)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Acceptance date
2017-02-23Publication date
2017-03-10Copyright date
2017Notes
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.analchem.6b04315.ISSN
0003-2700eISSN
1520-6882Publisher version
Language
- en